Ectoine accumulation in Brevibacterium epidermis

As a halotolerant bacterial species, Brevibacterium epidermis DSM 20659 can grow at relatively high salinity, tolerating up to 2 M NaCl. It synthesizes ectoine and the intracellular content increases with the medium salinity, with a maximum of 0.14 g ectoine/g CDW at 1 M NaCl. Sugar-stressed cells d...

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Veröffentlicht in:	Biotechnology letters 2004-10, Vol.26 (19), p.1481-1485
Hauptverfasser:	ONRAEDT, Annelies, DE MUYNCK, Cassandra, WALCARIUS, Bart, SOETAERT, Wim, VANDAMME, Erick
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids, Diamino - metabolism Bacteria Biological and medical sciences Biotechnology Brevibacterium - physiology Carbohydrate Metabolism Carbon - metabolism Carbon sources Cell Proliferation Epidermis Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - physiology Hydrogen-Ion Concentration Metabolic Clearance Rate Microbiology Osmotic Pressure Salinity Sodium chloride Water-Electrolyte Balance - physiology
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Beschreibung
Zusammenfassung:	As a halotolerant bacterial species, Brevibacterium epidermis DSM 20659 can grow at relatively high salinity, tolerating up to 2 M NaCl. It synthesizes ectoine and the intracellular content increases with the medium salinity, with a maximum of 0.14 g ectoine/g CDW at 1 M NaCl. Sugar-stressed cells do not synthesize ectoine. Ectoine synthesis is also affected by the presence of external osmolytes. Added betaine is taken up and completely replaced ectoine, while L-proline is only temporarily accumulated after which ectoine is synthesized. The strain can metabolize ectoine; L-glutamate is a better carbon source for ectoine synthesis than L-aspartate.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:BILE.0000044448.86907.e4