Monitoring the formation of biosilica catalysed by histidine-tagged silicatein
Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and s...
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Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2004-12 (24), p.2848-2849 |
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creator | Tahir, Muhammad Nawaz Théato, Patrick Müller, Werner E G Schröder, Heinz C Janshoff, Andreas Zhang, Jian Huth, Joachim Tremel, Wolfgang |
description | Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and scanning electron microscopy. |
doi_str_mv | 10.1039/b410283e |
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source | MEDLINE; Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
subjects | Catalysis Cathepsins - metabolism Histidine - metabolism Microscopy, Atomic Force Microscopy, Electron, Scanning Molecular Structure Silicon Dioxide - chemistry Silicon Dioxide - metabolism Surface Plasmon Resonance |
title | Monitoring the formation of biosilica catalysed by histidine-tagged silicatein |
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