Monitoring the formation of biosilica catalysed by histidine-tagged silicatein

Monitoring the formation of biosilica catalysed by histidine-tagged silicatein

Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and s...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2004-12 (24), p.2848-2849
Hauptverfasser: Tahir, Muhammad Nawaz, Théato, Patrick, Müller, Werner E G, Schröder, Heinz C, Janshoff, Andreas, Zhang, Jian, Huth, Joachim, Tremel, Wolfgang
Format: Artikel
Sprache:eng
Schlagworte:
Catalysis
Cathepsins - metabolism
Histidine - metabolism
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Molecular Structure
Silicon Dioxide - chemistry
Silicon Dioxide - metabolism
Surface Plasmon Resonance
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Beschreibung
Zusammenfassung:Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and scanning electron microscopy.
ISSN:1359-7345
1364-548X
DOI:10.1039/b410283e