Variation in the Levels of Inflammatory Cytokines Depending on Ischemic Time: Effects on Respiratory Variables

Abstract Objectives We sought to evaluate the association between ischemic times, cytokines—interleukin (IL)-6, IL-1b, tumor necrosis factor-alpha, sIL-2r, IL-8, and IL-10—and alterations in gaseous exchange. Materials and Methods This prospective study of 42 orthotopic liver transplantation (OLT) recipients examined ischemic times and respiratory variables measured as alterations in intrapulmonary shunt and in the P o2 /Fi o2 ratio. Centrifuged blood samples were frozen at −80°C for storage. The Inmulite-One system (Euro/Dpc, Gwynedd, UK) was used to determine the concentration of cytokines. For statistical analysis, we used the Pearson correlation coefficient. Results The average cold ischemic time was 478 minutes (range, 35–929) and warm ischemic time was 69.58 minutes (range, 20–180). The warm ischemic time affected the degree of shunt at the end of the operation ( P < .027) and the levels of IL-10 ( P < .018) and IL-6 ( P < .000). The final degree of shunting and IL-10 ( P < .044) showed a correlation. The cold ischemic time affected IL-1 ( P < .046) and IL-8 levels ( P < .023). The reperfusion syndrome was correlated with the final levels of IL-10 ( P < .064) and of IL-8 ( P < .066). Conclusion Warm and cold ischemic times affect the final cytokine levels and the degree of intrapulmonary shunt.