Hybridization of Oligonucleotide-Modified Silver and Gold Nanoparticles in Aqueous Dispersions and on Gold Films

Functionalization of silver and gold nanoparticles by 12mer-thiolated homo-oligonucleotides, SA and ST (containing only adenine or thymine, respectively), and their hybridization and dehybridization in aqueous dispersions have been described. In addition, ST and SA were self-assembled onto gold film...

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Veröffentlicht in:	Journal of the American Chemical Society 2004-12, Vol.126 (48), p.15784-15789
Hauptverfasser:	Tokareva, Iryna, Hutter, Eliza
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - chemistry Biological and medical sciences Chemistry Colloidal state and disperse state Disperse state. Micelles DNA - chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General and physical chemistry Gold - chemistry Microscopy, Electron, Transmission Molecular biophysics Nanostructures - chemistry Nucleic Acid Hybridization - methods Oligonucleotides - chemistry Physical and chemical studies. Granulometry. Electrokinetic phenomena Physico-chemical properties of biomolecules Silver - chemistry Spectroscopy, Fourier Transform Infrared - methods Sulfhydryl Compounds - chemistry Thymine - chemistry
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Beschreibung
Zusammenfassung:	Functionalization of silver and gold nanoparticles by 12mer-thiolated homo-oligonucleotides, SA and ST (containing only adenine or thymine, respectively), and their hybridization and dehybridization in aqueous dispersions have been described. In addition, ST and SA were self-assembled onto gold films and hybridized with their complementary pairs, unlabeled or labeled by gold and silver nanoparticles. The base pairing between DNA strands and the types of oligonucleotides (adenine or thymine) attached to the nanoparticles was detected by Polarization Modulated Fourier Transform Infrared Reflection Absorption Spectroscopy (PM-FTIRRAS).
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja046779k