Annoglabayin, a novel dimeric kaurane diterpenoid, and apoptosis in Hep G2 cells of an annomontacin from the fruits of Annona glabra

Annoglabayin (1), a novel Annona dimeric kaurane diterpenoid, has been isolated from Annona glabra, and its structure was determined on the basis of spectroscopic analysis. Annoglabayin (1) contains a unique carbon bridge between two nor-ent-kaurane monomeric units. The dose-response of 2 in Hep G2 cells indicated that 2 increased DNA damage. In addition, our results showed that 2 induced a noticeable decrease in mitochondrial transmembrane potential during treatment. These results indicate that 2 produces apoptotic events in Hep G2 cells, through inducing changes in mitochondria.