SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. I...

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Veröffentlicht in:	FEBS letters 2009-04, Vol.583 (8), p.1261-1266
Hauptverfasser:	Kumakura, Naoyoshi, Takeda, Atsushi, Fujioka, Yoichiro, Motose, Hiroyasu, Takano, Ryo, Watanabe, Yuichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	AGO Arabidopsis Arabidopsis Proteins - metabolism argonaute BiFC bimolecular fluorescent complementation Blotting, Northern Cytoplasmic Granules - metabolism DCL DCP1 decapping protein 1 Dicer-like 4 dicer-like enzyme Genetic Complementation Test GFP green fluorescent protein microRNA miRNA P-body Processing body RDR6 RNA Replicase - metabolism RNA silencing RNA, Small Interfering RNA-dependent RNA polymerase 6 SGS3 siRNA small-interfering RNA Suppressor of gene silencing 3 ta-siRNA trans-acting small-interfering RNA yellow fluorescent protein YFP
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