SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. I...

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Veröffentlicht in:	FEBS letters 2009-04, Vol.583 (8), p.1261-1266
Hauptverfasser:	Kumakura, Naoyoshi, Takeda, Atsushi, Fujioka, Yoichiro, Motose, Hiroyasu, Takano, Ryo, Watanabe, Yuichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	AGO Arabidopsis Arabidopsis Proteins - metabolism argonaute BiFC bimolecular fluorescent complementation Blotting, Northern Cytoplasmic Granules - metabolism DCL DCP1 decapping protein 1 Dicer-like 4 dicer-like enzyme Genetic Complementation Test GFP green fluorescent protein microRNA miRNA P-body Processing body RDR6 RNA Replicase - metabolism RNA silencing RNA, Small Interfering RNA-dependent RNA polymerase 6 SGS3 siRNA small-interfering RNA Suppressor of gene silencing 3 ta-siRNA trans-acting small-interfering RNA yellow fluorescent protein YFP
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Zusammenfassung:	Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. MINT- 7014710: SGS3 (uniprotkb: Q9LDX1) and RDR6 (uniprotkb: Q9SG02) physically interact (MI: 0218) by bimolecular fluorescence complementation (MI: 0809) MINT- 7014697: RDR6 (uniprotkb: Q9SG02) and SGS3 (uniprotkb: Q9LDX1) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416)
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2009.03.055