Identification and characterization of zinc-starvation-induced ZIP transporters from barley roots

Zinc (Zn) is an essential element for plants but limited information is currently available on the molecular basis for Zn 2+ transport in crop species. To expand the knowledge on Zn 2+ transport in barley ( Hordeum vulgare L.), a cDNA library prepared from barley roots was expressed in the yeast ( Saccharomyces cerevisiae) mutant strain Δzrt1/ Δzrt2, defective in Zn 2+ uptake. This strategy resulted in isolation and identification of three new Zn 2+ transporters from barley. All of the predicted proteins have a high similarity to the ZIP protein family, and are designated HvZIP3, HvZIP5 and HvZIP8, respectively. Complementation studies in Δzrt1/ Δzrt2 showed restored growth of the yeast cells transformed with the different HvZIPs, although with different efficiency. Transformation into Fe 2+ and Mn 2+ uptake defective yeast mutants showed that the HvZIPs were unable to restore the growth on Fe 2+ and Mn 2+ limited media, respectively, indicating a specific role in Zn 2+ transport. In intact barley roots, HvZIP8 was constitutively expressed whereas HvZIP3 and HvZIP5 were mainly expressed in −Zn plants. These results suggest that HvZIP3, HvZIP5 and HvZIP8 are Zn 2+ transporters involved in Zn 2+ homeostasis in barley roots. The new transporters may facilitate breeding of barley genotypes with improved Zn efficiency and Zn content.