Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses

Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 mer YEDVNGIRK (NP 165-173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various...

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Veröffentlicht in:	Archives of virology 2004-12, Vol.149 (12), p.2427-2434
Hauptverfasser:	OKUMURA, M, YOSHIMATSU, K, ARAKI, K, LEE, B.-H, ASANO, A, AGUI, T, ARIKAWA, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Monoclonal - immunology Antibodies, Viral - chemistry Antibodies, Viral - immunology Antigens Biological and medical sciences Enzymes Epitopes - chemistry Epitopes - immunology Fundamental and applied biological sciences. Psychology Hantavirus Infections Microbiology Miscellaneous Molecular Sequence Data Monoclonal antibodies Nucleocapsid - chemistry Nucleocapsid - immunology Nucleocapsid Proteins Orthohantavirus - chemistry Orthohantavirus - immunology Peptides Proteins Serology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Veterinary colleges Veterinary medicine Virology Viruses
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Zusammenfassung:	Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 mer YEDVNGIRK (NP 165-173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various hantavirus sequences bound MAb E5/G6 consistently, despite the existence of several amino acid variations in this region. These results indicate that MAb E5/G6 is a useful tool for detecting hantavirus antigen in rodent or patient tissues using Western blotting or other immunohistochemical assays.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-004-0393-9