Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis
Abstract The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, iso...
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| Veröffentlicht in: | FEMS microbiology letters 2004-12, Vol.241 (1), p.57-65 |
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| creator | Moran, Anthony P. Shiberu, Bethlehem Ferris, John A. Knirel, Yuriy A. Senchenkova, Sof'ya N. Perepelov, Andreij V. Jansson, Per-Erik Goldberg, Joanna B. |
| description | Abstract
The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase. |
| doi_str_mv | 10.1016/j.femsle.2004.10.004 |
| format | Article |
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The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2004.10.004</identifier><identifier>PMID: 15556710</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Core oligosaccharide ; Epitopes ; Genes ; Genes, Bacterial - physiology ; Genomes ; Glucosyltransferase ; Glucosyltransferases - genetics ; Glycosyltransferase ; Helicobacter pylori ; Helicobacter pylori - genetics ; Helicobacter pylori - metabolism ; Homology ; Immunoblotting ; Lewis antigen ; Lipopolysaccharide ; Lipopolysaccharides ; Lipopolysaccharides - biosynthesis ; Lipopolysaccharides - chemistry ; Microbiology ; Structural analysis ; Synthesis</subject><ispartof>FEMS microbiology letters, 2004-12, Vol.241 (1), p.57-65</ispartof><rights>2004 Federation of European Microbiological Societies 2004</rights><rights>2004 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4507-99738770b5afb94c72260044dbb1b82c5fdd57e704ea202381b86006e5b537563</citedby><cites>FETCH-LOGICAL-c4507-99738770b5afb94c72260044dbb1b82c5fdd57e704ea202381b86006e5b537563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.femsle.2004.10.004$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.femsle.2004.10.004$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15556710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moran, Anthony P.</creatorcontrib><creatorcontrib>Shiberu, Bethlehem</creatorcontrib><creatorcontrib>Ferris, John A.</creatorcontrib><creatorcontrib>Knirel, Yuriy A.</creatorcontrib><creatorcontrib>Senchenkova, Sof'ya N.</creatorcontrib><creatorcontrib>Perepelov, Andreij V.</creatorcontrib><creatorcontrib>Jansson, Per-Erik</creatorcontrib><creatorcontrib>Goldberg, Joanna B.</creatorcontrib><title>Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase.</description><subject>Core oligosaccharide</subject><subject>Epitopes</subject><subject>Genes</subject><subject>Genes, Bacterial - physiology</subject><subject>Genomes</subject><subject>Glucosyltransferase</subject><subject>Glucosyltransferases - genetics</subject><subject>Glycosyltransferase</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - metabolism</subject><subject>Homology</subject><subject>Immunoblotting</subject><subject>Lewis antigen</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - biosynthesis</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Microbiology</subject><subject>Structural analysis</subject><subject>Synthesis</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkVFrFDEUhYNY7Nr6D0QCgujDbJPMJHfmUYp1lS0toi--hEzmjs2SnUyTHWT-vVlmQfDFPl04fOdwLoeQ15ytOePqarfucZ88rgVjVZbW-TwjKy6hKlSj6udkxUqoC84aOCcvU9qxTAimXpBzLqVUwNmK_PwWPNLQ0w16Z0Nr7AEjHWcfoqOxN1_pLxww0febe8ZlQ83Q0c09r7j6QN1AvRvDGPycjLUPJroOaZqHwwMmly7JWW98wlene0F-3Hz6fr0ptnefv1x_3Ba2kgyKpoGyBmCtNH3bVBaEULlo1bUtb2thZd91EhBYhUYwUdZZzYBC2coSpCovyLsld4zhccJ00HuXLHpvBgxT0gpYoyrR_BfkUJeM15DBt_-AuzDFIT-hRcmAK1AgMlUtlI0hpYi9HqPbmzhrzvRxIr3Ty0T6ONFRzSfb3pzCp3aP3V_TaZMMwAL8dh7nJ4Xqm9utPPa-WpxhGp9W5g9i-6uD</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Moran, Anthony P.</creator><creator>Shiberu, Bethlehem</creator><creator>Ferris, John A.</creator><creator>Knirel, Yuriy A.</creator><creator>Senchenkova, Sof'ya N.</creator><creator>Perepelov, Andreij V.</creator><creator>Jansson, Per-Erik</creator><creator>Goldberg, Joanna B.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis</title><author>Moran, Anthony P. ; Shiberu, Bethlehem ; Ferris, John A. ; Knirel, Yuriy A. ; Senchenkova, Sof'ya N. ; Perepelov, Andreij V. ; Jansson, Per-Erik ; Goldberg, Joanna B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4507-99738770b5afb94c72260044dbb1b82c5fdd57e704ea202381b86006e5b537563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Core oligosaccharide</topic><topic>Epitopes</topic><topic>Genes</topic><topic>Genes, Bacterial - physiology</topic><topic>Genomes</topic><topic>Glucosyltransferase</topic><topic>Glucosyltransferases - genetics</topic><topic>Glycosyltransferase</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - metabolism</topic><topic>Homology</topic><topic>Immunoblotting</topic><topic>Lewis antigen</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - biosynthesis</topic><topic>Lipopolysaccharides - chemistry</topic><topic>Microbiology</topic><topic>Structural analysis</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moran, Anthony P.</creatorcontrib><creatorcontrib>Shiberu, Bethlehem</creatorcontrib><creatorcontrib>Ferris, John A.</creatorcontrib><creatorcontrib>Knirel, Yuriy A.</creatorcontrib><creatorcontrib>Senchenkova, Sof'ya N.</creatorcontrib><creatorcontrib>Perepelov, Andreij V.</creatorcontrib><creatorcontrib>Jansson, Per-Erik</creatorcontrib><creatorcontrib>Goldberg, Joanna B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moran, Anthony P.</au><au>Shiberu, Bethlehem</au><au>Ferris, John A.</au><au>Knirel, Yuriy A.</au><au>Senchenkova, Sof'ya N.</au><au>Perepelov, Andreij V.</au><au>Jansson, Per-Erik</au><au>Goldberg, Joanna B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>241</volume><issue>1</issue><spage>57</spage><epage>65</epage><pages>57-65</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15556710</pmid><doi>10.1016/j.femsle.2004.10.004</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Core oligosaccharide Epitopes Genes Genes, Bacterial - physiology Genomes Glucosyltransferase Glucosyltransferases - genetics Glycosyltransferase Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - metabolism Homology Immunoblotting Lewis antigen Lipopolysaccharide Lipopolysaccharides Lipopolysaccharides - biosynthesis Lipopolysaccharides - chemistry Microbiology Structural analysis Synthesis |
| title | Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis |
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