Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis

Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis

Abstract The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, iso...

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Veröffentlicht in:FEMS microbiology letters 2004-12, Vol.241 (1), p.57-65
Hauptverfasser: Moran, Anthony P., Shiberu, Bethlehem, Ferris, John A., Knirel, Yuriy A., Senchenkova, Sof'ya N., Perepelov, Andreij V., Jansson, Per-Erik, Goldberg, Joanna B.
Format: Artikel
Sprache:eng
Schlagworte:
Core oligosaccharide
Epitopes
Genes
Genes, Bacterial - physiology
Genomes
Glucosyltransferase
Glucosyltransferases - genetics
Glycosyltransferase
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - metabolism
Homology
Immunoblotting
Lewis antigen
Lipopolysaccharide
Lipopolysaccharides
Lipopolysaccharides - biosynthesis
Lipopolysaccharides - chemistry
Microbiology
Structural analysis
Synthesis
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Zusammenfassung:Abstract The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.10.004