UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms
P2Y receptors have been reported to modulate gastrointestinal functions. The newest family member is the nucleotide-sugar receptor P2Y14. P2ry14 mRNA was detected throughout the rat gut, with the highest level being in the forestomach. We investigated the role of the receptor in stomach motility usi...
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| Veröffentlicht in: | American journal of physiology: Gastrointestinal and liver physiology 2009-04, Vol.296 (4), p.G923-G930 |
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| container_title | American journal of physiology: Gastrointestinal and liver physiology |
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| creator | Bassil, Anna K Bourdu, Sophie Townson, Karen A Wheeldon, Alan Jarvie, Emma M Zebda, Noureddine Abuin, Alejandro Grau, Evelyn Livi, George P Punter, Lorraine Latcham, Judith Grimes, Angela M Hurp, David P Downham, Kelly M Sanger, Gareth J Winchester, Wendy J Morrison, Alastair D Moore, Gary B T |
| description | P2Y receptors have been reported to modulate gastrointestinal functions. The newest family member is the nucleotide-sugar receptor P2Y14. P2ry14 mRNA was detected throughout the rat gut, with the highest level being in the forestomach. We investigated the role of the receptor in stomach motility using cognate agonists and knockout (KO) mice. In rat isolated forestomach, 100 microM UDP-glucose and 100 muM UDP-galactose both increased the baseline muscle tension (BMT) by 6.2+/-0.6 and 1.6+/-0.6 mN (P |
| doi_str_mv | 10.1152/ajpgi.90363.2008 |
| format | Article |
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The newest family member is the nucleotide-sugar receptor P2Y14. P2ry14 mRNA was detected throughout the rat gut, with the highest level being in the forestomach. We investigated the role of the receptor in stomach motility using cognate agonists and knockout (KO) mice. In rat isolated forestomach, 100 microM UDP-glucose and 100 muM UDP-galactose both increased the baseline muscle tension (BMT) by 6.2+/-0.6 and 1.6+/-0.6 mN (P<0.05, n=3-4), respectively, and the amplitude of contractions during electrical field stimulation (EFS) by 3.7+/-1.7 and 4.3+/-2.5 mN (P<0.05, n=3-4), respectively. In forestomach from wild-type (WT) mice, 100 microM UDP-glucose increased the BMT by 1.0+/-0.1 mN (P<0.05, n=6) but this effect was lost in the KO mice (change of -0.1+/-0.1 mN, n=6). The 100 microM UDP-glucose also increased the contraction amplitude during EFS in this tissue from the WT animals (0.9+/-0.4 mN, P < 0.05, n=6) but not from the KO mice (0.0+/-0.2 mN, n=6). In vivo, UDP-glucose at 2,000 mg/kg ip reduced gastric emptying in rats by 49.7% (P<0.05, n=4-6) and in WT and KO mice by 56.1 and 66.2%, respectively (P<0.05, n=7-10) vs. saline-treated control animals. There was no significant difference in gastric emptying between WT and KO animals receiving either saline or d-glucose. These results demonstrate a novel function of the P2Y14 receptor associated with contractility in the rodent stomach that does not lead to altered gastric emptying after receptor deletion and an ability of UDP-glucose to delay gastric emptying without involving the P2Y14 receptor.]]></description><identifier>ISSN: 0193-1857</identifier><identifier>DOI: 10.1152/ajpgi.90363.2008</identifier><identifier>PMID: 19164486</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Dose-Response Relationship, Drug ; Gastric Emptying - drug effects ; Gene Expression Regulation - physiology ; Lac Operon - genetics ; Lac Operon - physiology ; Mice ; Mice, Knockout ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Rats ; Receptors, Purinergic P2 - genetics ; Receptors, Purinergic P2 - metabolism ; Receptors, Purinergic P2Y ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Uridine Diphosphate Galactose - pharmacology ; Uridine Diphosphate Glucose - pharmacology</subject><ispartof>American journal of physiology: Gastrointestinal and liver physiology, 2009-04, Vol.296 (4), p.G923-G930</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19164486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bassil, Anna K</creatorcontrib><creatorcontrib>Bourdu, Sophie</creatorcontrib><creatorcontrib>Townson, Karen A</creatorcontrib><creatorcontrib>Wheeldon, Alan</creatorcontrib><creatorcontrib>Jarvie, Emma M</creatorcontrib><creatorcontrib>Zebda, Noureddine</creatorcontrib><creatorcontrib>Abuin, Alejandro</creatorcontrib><creatorcontrib>Grau, Evelyn</creatorcontrib><creatorcontrib>Livi, George P</creatorcontrib><creatorcontrib>Punter, Lorraine</creatorcontrib><creatorcontrib>Latcham, Judith</creatorcontrib><creatorcontrib>Grimes, Angela M</creatorcontrib><creatorcontrib>Hurp, David P</creatorcontrib><creatorcontrib>Downham, Kelly M</creatorcontrib><creatorcontrib>Sanger, Gareth J</creatorcontrib><creatorcontrib>Winchester, Wendy J</creatorcontrib><creatorcontrib>Morrison, Alastair D</creatorcontrib><creatorcontrib>Moore, Gary B T</creatorcontrib><title>UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms</title><title>American journal of physiology: Gastrointestinal and liver physiology</title><addtitle>Am J Physiol Gastrointest Liver Physiol</addtitle><description><![CDATA[P2Y receptors have been reported to modulate gastrointestinal functions. The newest family member is the nucleotide-sugar receptor P2Y14. P2ry14 mRNA was detected throughout the rat gut, with the highest level being in the forestomach. We investigated the role of the receptor in stomach motility using cognate agonists and knockout (KO) mice. In rat isolated forestomach, 100 microM UDP-glucose and 100 muM UDP-galactose both increased the baseline muscle tension (BMT) by 6.2+/-0.6 and 1.6+/-0.6 mN (P<0.05, n=3-4), respectively, and the amplitude of contractions during electrical field stimulation (EFS) by 3.7+/-1.7 and 4.3+/-2.5 mN (P<0.05, n=3-4), respectively. In forestomach from wild-type (WT) mice, 100 microM UDP-glucose increased the BMT by 1.0+/-0.1 mN (P<0.05, n=6) but this effect was lost in the KO mice (change of -0.1+/-0.1 mN, n=6). The 100 microM UDP-glucose also increased the contraction amplitude during EFS in this tissue from the WT animals (0.9+/-0.4 mN, P < 0.05, n=6) but not from the KO mice (0.0+/-0.2 mN, n=6). In vivo, UDP-glucose at 2,000 mg/kg ip reduced gastric emptying in rats by 49.7% (P<0.05, n=4-6) and in WT and KO mice by 56.1 and 66.2%, respectively (P<0.05, n=7-10) vs. saline-treated control animals. There was no significant difference in gastric emptying between WT and KO animals receiving either saline or d-glucose. These results demonstrate a novel function of the P2Y14 receptor associated with contractility in the rodent stomach that does not lead to altered gastric emptying after receptor deletion and an ability of UDP-glucose to delay gastric emptying without involving the P2Y14 receptor.]]></description><subject>Animals</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gastric Emptying - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Lac Operon - genetics</subject><subject>Lac Operon - physiology</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Rats</subject><subject>Receptors, Purinergic P2 - genetics</subject><subject>Receptors, Purinergic P2 - metabolism</subject><subject>Receptors, Purinergic P2Y</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Uridine Diphosphate Galactose - pharmacology</subject><subject>Uridine Diphosphate Glucose - pharmacology</subject><issn>0193-1857</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAURT2AaCnsTMgTW8p7ju3EIyqfUiU60IEpcu3XNFXihNgZ-PcgAWI60tXRHQ5jVwhLRCVu7XGom6WBXOdLAVCesDmgyTMsVTFj5zEeAUAJxDM2Q4NaylLPmdveb7K6nVwfiXe9n1qbKPLaxjQ2ju-n4FLTB54OYz_VB74R7yj5SI6G1I-Zp4GCp5C4DZ5nTfgfOnIHG5rYxQt2urdtpMtfLtj28eFt9ZytX59eVnfrbEAhU1YWiDupwSN6MhpBGiS3016Vhd57X5AUSnlnSg_oBBRS5BaUtNIiKSPzBbv5-R3G_mOimKquiY7a1gbqp1jpAkyuQH-L17_itOvIV8PYdHb8rP6y5F_STWOT</recordid><startdate>200904</startdate><enddate>200904</enddate><creator>Bassil, Anna K</creator><creator>Bourdu, Sophie</creator><creator>Townson, Karen A</creator><creator>Wheeldon, Alan</creator><creator>Jarvie, Emma M</creator><creator>Zebda, Noureddine</creator><creator>Abuin, Alejandro</creator><creator>Grau, Evelyn</creator><creator>Livi, George P</creator><creator>Punter, Lorraine</creator><creator>Latcham, Judith</creator><creator>Grimes, Angela M</creator><creator>Hurp, David P</creator><creator>Downham, Kelly M</creator><creator>Sanger, Gareth J</creator><creator>Winchester, Wendy J</creator><creator>Morrison, Alastair D</creator><creator>Moore, Gary B T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200904</creationdate><title>UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms</title><author>Bassil, Anna K ; Bourdu, Sophie ; Townson, Karen A ; Wheeldon, Alan ; Jarvie, Emma M ; Zebda, Noureddine ; Abuin, Alejandro ; Grau, Evelyn ; Livi, George P ; Punter, Lorraine ; Latcham, Judith ; Grimes, Angela M ; Hurp, David P ; Downham, Kelly M ; Sanger, Gareth J ; Winchester, Wendy J ; Morrison, Alastair D ; Moore, Gary B T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-8711b460d11de9610491ecb6d5876fdd7e4255dc98d01c207423a054a4a1e5943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gastric Emptying - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Lac Operon - genetics</topic><topic>Lac Operon - physiology</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Rats</topic><topic>Receptors, Purinergic P2 - genetics</topic><topic>Receptors, Purinergic P2 - metabolism</topic><topic>Receptors, Purinergic P2Y</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Uridine Diphosphate Galactose - pharmacology</topic><topic>Uridine Diphosphate Glucose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bassil, Anna K</creatorcontrib><creatorcontrib>Bourdu, Sophie</creatorcontrib><creatorcontrib>Townson, Karen A</creatorcontrib><creatorcontrib>Wheeldon, Alan</creatorcontrib><creatorcontrib>Jarvie, Emma M</creatorcontrib><creatorcontrib>Zebda, Noureddine</creatorcontrib><creatorcontrib>Abuin, Alejandro</creatorcontrib><creatorcontrib>Grau, Evelyn</creatorcontrib><creatorcontrib>Livi, George P</creatorcontrib><creatorcontrib>Punter, Lorraine</creatorcontrib><creatorcontrib>Latcham, Judith</creatorcontrib><creatorcontrib>Grimes, Angela M</creatorcontrib><creatorcontrib>Hurp, David P</creatorcontrib><creatorcontrib>Downham, Kelly M</creatorcontrib><creatorcontrib>Sanger, Gareth J</creatorcontrib><creatorcontrib>Winchester, Wendy J</creatorcontrib><creatorcontrib>Morrison, Alastair D</creatorcontrib><creatorcontrib>Moore, Gary B T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bassil, Anna K</au><au>Bourdu, Sophie</au><au>Townson, Karen A</au><au>Wheeldon, Alan</au><au>Jarvie, Emma M</au><au>Zebda, Noureddine</au><au>Abuin, Alejandro</au><au>Grau, Evelyn</au><au>Livi, George P</au><au>Punter, Lorraine</au><au>Latcham, Judith</au><au>Grimes, Angela M</au><au>Hurp, David P</au><au>Downham, Kelly M</au><au>Sanger, Gareth J</au><au>Winchester, Wendy J</au><au>Morrison, Alastair D</au><au>Moore, Gary B T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms</atitle><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle><addtitle>Am J Physiol Gastrointest Liver Physiol</addtitle><date>2009-04</date><risdate>2009</risdate><volume>296</volume><issue>4</issue><spage>G923</spage><epage>G930</epage><pages>G923-G930</pages><issn>0193-1857</issn><abstract><![CDATA[P2Y receptors have been reported to modulate gastrointestinal functions. The newest family member is the nucleotide-sugar receptor P2Y14. P2ry14 mRNA was detected throughout the rat gut, with the highest level being in the forestomach. We investigated the role of the receptor in stomach motility using cognate agonists and knockout (KO) mice. In rat isolated forestomach, 100 microM UDP-glucose and 100 muM UDP-galactose both increased the baseline muscle tension (BMT) by 6.2+/-0.6 and 1.6+/-0.6 mN (P<0.05, n=3-4), respectively, and the amplitude of contractions during electrical field stimulation (EFS) by 3.7+/-1.7 and 4.3+/-2.5 mN (P<0.05, n=3-4), respectively. In forestomach from wild-type (WT) mice, 100 microM UDP-glucose increased the BMT by 1.0+/-0.1 mN (P<0.05, n=6) but this effect was lost in the KO mice (change of -0.1+/-0.1 mN, n=6). The 100 microM UDP-glucose also increased the contraction amplitude during EFS in this tissue from the WT animals (0.9+/-0.4 mN, P < 0.05, n=6) but not from the KO mice (0.0+/-0.2 mN, n=6). In vivo, UDP-glucose at 2,000 mg/kg ip reduced gastric emptying in rats by 49.7% (P<0.05, n=4-6) and in WT and KO mice by 56.1 and 66.2%, respectively (P<0.05, n=7-10) vs. saline-treated control animals. There was no significant difference in gastric emptying between WT and KO animals receiving either saline or d-glucose. These results demonstrate a novel function of the P2Y14 receptor associated with contractility in the rodent stomach that does not lead to altered gastric emptying after receptor deletion and an ability of UDP-glucose to delay gastric emptying without involving the P2Y14 receptor.]]></abstract><cop>United States</cop><pmid>19164486</pmid><doi>10.1152/ajpgi.90363.2008</doi></addata></record> |
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| ispartof | American journal of physiology: Gastrointestinal and liver physiology, 2009-04, Vol.296 (4), p.G923-G930 |
| issn | 0193-1857 |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Dose-Response Relationship, Drug Gastric Emptying - drug effects Gene Expression Regulation - physiology Lac Operon - genetics Lac Operon - physiology Mice Mice, Knockout Muscle Contraction - drug effects Muscle, Smooth - drug effects Rats Receptors, Purinergic P2 - genetics Receptors, Purinergic P2 - metabolism Receptors, Purinergic P2Y RNA, Messenger - genetics RNA, Messenger - metabolism Uridine Diphosphate Galactose - pharmacology Uridine Diphosphate Glucose - pharmacology |
| title | UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms |
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