The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting...

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Veröffentlicht in:BMB reports 2009-03, Vol.42 (3), p.154-159
Hauptverfasser: Zhou, Wu (Zhejiang University, Hangzhou, China), Chen, Haixiang (Zhejiang University, Hangzhou, China), Zhang, Lihuang (Zhejiang University, Hangzhou, China), E-mail: imm@zju.edu.cn
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Sprache:eng
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Zusammenfassung:JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor.
ISSN:1976-6696
DOI:10.5483/BMBRep.2009.42.3.154