Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts
The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine...
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| Veröffentlicht in: | Histochemistry and cell biology 2009-05, Vol.131 (5), p.583-591 |
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| creator | Sato, Shigehisa Tsuchiya, Masahiro Komaki, Ken-ichiro Kusunoki, Shin-ichiro Tsuchiya, Shinobu Haruyama, Naoto Takahashi, Ichiro Sasano, Yasuyuki Watanabe, Makoto |
| description | The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function. |
| doi_str_mv | 10.1007/s00418-009-0556-6 |
| format | Article |
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Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-009-0556-6</identifier><identifier>PMID: 19156436</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Aging ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cellular biology ; Collagen Type I - metabolism ; Dentin - cytology ; Dentin - metabolism ; Dentinogenesis ; Developmental Biology ; Odontoblasts - cytology ; Odontoblasts - metabolism ; Original Paper ; Osteopontin - metabolism ; Protein Transport ; Proteins ; Rats ; Rats, Wistar ; Ribonucleic acid ; RNA ; Rodents</subject><ispartof>Histochemistry and cell biology, 2009-05, Vol.131 (5), p.583-591</ispartof><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-523405c0a29d6f2bd6ac9205c0bec31a5d2c40bac266bb0d54b064543715f1503</citedby><cites>FETCH-LOGICAL-c490t-523405c0a29d6f2bd6ac9205c0bec31a5d2c40bac266bb0d54b064543715f1503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00418-009-0556-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00418-009-0556-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19156436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sato, Shigehisa</creatorcontrib><creatorcontrib>Tsuchiya, Masahiro</creatorcontrib><creatorcontrib>Komaki, Ken-ichiro</creatorcontrib><creatorcontrib>Kusunoki, Shin-ichiro</creatorcontrib><creatorcontrib>Tsuchiya, Shinobu</creatorcontrib><creatorcontrib>Haruyama, Naoto</creatorcontrib><creatorcontrib>Takahashi, Ichiro</creatorcontrib><creatorcontrib>Sasano, Yasuyuki</creatorcontrib><creatorcontrib>Watanabe, Makoto</creatorcontrib><title>Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function.</description><subject>Aging</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cellular biology</subject><subject>Collagen Type I - metabolism</subject><subject>Dentin - cytology</subject><subject>Dentin - metabolism</subject><subject>Dentinogenesis</subject><subject>Developmental Biology</subject><subject>Odontoblasts - cytology</subject><subject>Odontoblasts - metabolism</subject><subject>Original Paper</subject><subject>Osteopontin - metabolism</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Rodents</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk9v1DAQxS0EokvhA3ABiwO3wIz_ZX1EVYFKlTiUStwsx7GXVFk72M5hvz2JsqISBzh5NPq9Z4_fEPIa4QMCtB8LgMB9A6AbkFI16gnZoeCsQdQ_npIdaLFv1NK5IC9KeQBAqRl7Ti5Qo1SCqx3Jd6dYf_oyFGpjT4dYs3V-HOfRZrrUsUwpV1uHFGkKtJ4mT2_olJNL42gPPtJ-zkM80DBHt1J2pP0Qgs8-1uGPLvUp1tSNttTykjwLdiz-1fm8JPefr79ffW1uv325ufp02zihoTaScQHSgWW6V4F1vbJOs7XTecfRyp45AZ11TKmug16KDpSQgrcoA0rgl-T95ru89tfsSzXHoayz2ejTXIxqQaMG9l-QIbYt7tUCvvsLfEhzXkZeGYmqbSVfINwgl1Mp2Qcz5eFo88kgmDU2s8VmltjMGptZjd-cjefu6PtHxTmnBWAbUKb1t31-vPlfrm83UbDJ2EMeirm_Y4AccFmKtt3z375LrYw</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Sato, Shigehisa</creator><creator>Tsuchiya, Masahiro</creator><creator>Komaki, Ken-ichiro</creator><creator>Kusunoki, Shin-ichiro</creator><creator>Tsuchiya, Shinobu</creator><creator>Haruyama, Naoto</creator><creator>Takahashi, Ichiro</creator><creator>Sasano, Yasuyuki</creator><creator>Watanabe, Makoto</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts</title><author>Sato, Shigehisa ; Tsuchiya, Masahiro ; Komaki, Ken-ichiro ; Kusunoki, Shin-ichiro ; Tsuchiya, Shinobu ; Haruyama, Naoto ; Takahashi, Ichiro ; Sasano, Yasuyuki ; Watanabe, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-523405c0a29d6f2bd6ac9205c0bec31a5d2c40bac266bb0d54b064543715f1503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cellular biology</topic><topic>Collagen Type I - metabolism</topic><topic>Dentin - cytology</topic><topic>Dentin - metabolism</topic><topic>Dentinogenesis</topic><topic>Developmental Biology</topic><topic>Odontoblasts - cytology</topic><topic>Odontoblasts - metabolism</topic><topic>Original Paper</topic><topic>Osteopontin - metabolism</topic><topic>Protein Transport</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Shigehisa</creatorcontrib><creatorcontrib>Tsuchiya, Masahiro</creatorcontrib><creatorcontrib>Komaki, Ken-ichiro</creatorcontrib><creatorcontrib>Kusunoki, Shin-ichiro</creatorcontrib><creatorcontrib>Tsuchiya, Shinobu</creatorcontrib><creatorcontrib>Haruyama, Naoto</creatorcontrib><creatorcontrib>Takahashi, Ichiro</creatorcontrib><creatorcontrib>Sasano, Yasuyuki</creatorcontrib><creatorcontrib>Watanabe, Makoto</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Shigehisa</au><au>Tsuchiya, Masahiro</au><au>Komaki, Ken-ichiro</au><au>Kusunoki, Shin-ichiro</au><au>Tsuchiya, Shinobu</au><au>Haruyama, Naoto</au><au>Takahashi, Ichiro</au><au>Sasano, Yasuyuki</au><au>Watanabe, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts</atitle><jtitle>Histochemistry and cell biology</jtitle><stitle>Histochem Cell Biol</stitle><addtitle>Histochem Cell Biol</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>131</volume><issue>5</issue><spage>583</spage><epage>591</epage><pages>583-591</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19156436</pmid><doi>10.1007/s00418-009-0556-6</doi><tpages>9</tpages></addata></record> |
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| subjects | Aging Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Cellular biology Collagen Type I - metabolism Dentin - cytology Dentin - metabolism Dentinogenesis Developmental Biology Odontoblasts - cytology Odontoblasts - metabolism Original Paper Osteopontin - metabolism Protein Transport Proteins Rats Rats, Wistar Ribonucleic acid RNA Rodents |
| title | Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts |
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