Synthesis of a Fluorogenic Analogue of Sphingosine-1-Phosphate and Its Use to Determine Sphingosine-1-Phosphate Lyase Activity

Illuminating an ER enzyme: We report on the design and synthesis of a fluorogenic chemical sensor (1) to measure sphingosine-1-phosphate lyase activity in high-throughput screening formats, as well as its validation using lyase knockout (Sgpl1-/-) cells.

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Bibliographische Detailangaben
Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2009-03, Vol.10 (5), p.820-822
Hauptverfasser:	Bedia, Carmen, Camacho, Luz, Casas, Josefina, Abad, José Luis, Delgado, Antonio, Van Veldhoven, Paul P, Fabriàs, Gemma
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde-Lyases - metabolism Animals Cells, Cultured enzymes Fibroblasts - cytology Fibroblasts - physiology fluorescence Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry high‐throughput screening Lysophospholipids - chemistry Mice Mice, Knockout Molecular Structure retro reactions sphingolipids Sphingosine - analogs & derivatives Sphingosine - chemistry
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Beschreibung
Zusammenfassung:	Illuminating an ER enzyme: We report on the design and synthesis of a fluorogenic chemical sensor (1) to measure sphingosine-1-phosphate lyase activity in high-throughput screening formats, as well as its validation using lyase knockout (Sgpl1-/-) cells.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200800809