Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis)

Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis)

A mapping referential family (F(1)) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage gro...

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Veröffentlicht in:Marine biotechnology (New York, N.Y.) N.Y.), 2004-08, Vol.6 (4), p.327-334
Hauptverfasser: Watanabe, Tomohisa, Fujita, Hironobu, Yamasaki, Kimio, Seki, Shingo, Taniguchi, Nobuhiko
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Aquaculture - methods
Chromosome Mapping
Deoxyribonucleic acid
DNA
Gene mapping
Genetic linkage
Genetics
Genomes
Microsatellite Repeats - genetics
Nucleic Acid Amplification Techniques
Osmeriformes - genetics
Plecoglossus altivelis
Polymorphism, Restriction Fragment Length
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Zusammenfassung:A mapping referential family (F(1)) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-003-0026-4