Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection
There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2004-11, Vol.52 (23), p.6875-6878 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Lodge-Ivey, Shanna L May, Tammy Petersen, Mark K Strickland, James R |
| description | There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at −20 °C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 μg/mL was calculated. The recoveries were >80%, and the coefficients of variation were |
| doi_str_mv | 10.1021/jf049736w |
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The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at −20 °C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 μg/mL was calculated. The recoveries were >80%, and the coefficients of variation were <15%. This method has proven to be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collected from cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced by ruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxal concentration has the potential to be a useful marker to assess ruminal nitrogen status to aid in more accurate diet formulation. Keywords: Methylglyoxal; HPLC; ruminants; nitrogen, carbohydrate</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf049736w</identifier><identifier>PMID: 15537289</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>analytical methods ; Animals ; Biological and medical sciences ; Body Fluids - chemistry ; Cattle ; Chromatography, High Pressure Liquid - methods ; Dietary Proteins - administration & dosage ; fluorescence ; fluorometry ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; high performance liquid chromatography ; Methods of analysis, processing and quality control, regulation, standards ; methylglyoxal ; Pyruvaldehyde - analysis ; Rumen ; rumen fluids ; toxic substances</subject><ispartof>Journal of agricultural and food chemistry, 2004-11, Vol.52 (23), p.6875-6878</ispartof><rights>Copyright © 2004 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a403t-689e38e250bd261d4d74b3b9a7d90f84ec45b2662be9d735496ccab939ed5a3f3</citedby><cites>FETCH-LOGICAL-a403t-689e38e250bd261d4d74b3b9a7d90f84ec45b2662be9d735496ccab939ed5a3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf049736w$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf049736w$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16277118$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15537289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lodge-Ivey, Shanna L</creatorcontrib><creatorcontrib>May, Tammy</creatorcontrib><creatorcontrib>Petersen, Mark K</creatorcontrib><creatorcontrib>Strickland, James R</creatorcontrib><title>Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at −20 °C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 μg/mL was calculated. The recoveries were >80%, and the coefficients of variation were <15%. This method has proven to be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collected from cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced by ruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxal concentration has the potential to be a useful marker to assess ruminal nitrogen status to aid in more accurate diet formulation. Keywords: Methylglyoxal; HPLC; ruminants; nitrogen, carbohydrate</description><subject>analytical methods</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Fluids - chemistry</subject><subject>Cattle</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dietary Proteins - administration & dosage</subject><subject>fluorescence</subject><subject>fluorometry</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>high performance liquid chromatography</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>methylglyoxal</subject><subject>Pyruvaldehyde - analysis</subject><subject>Rumen</subject><subject>rumen fluids</subject><subject>toxic substances</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc2O0zAUhS0EYsrAghcAb0BiEfBPbMdL1GEoUhDVzBSxsxzHaV2SuGMnMHl7HLWablhZ8vl8zvW5ALzG6CNGBH_aNyiXgvK_T8ACM4IyhnHxFCxQErOCcXwBXsS4RwgVTKDn4AIzRgUp5AL8ubKDDZ3r9eB8D30Dv9thN7XbdvIPuoWuhzfjLLfwuh1dDasJrtx2l61taHzodG8sLN39LC13wXd68NugD7sJbqLrt_Mrn67tEJyBc5iZg16CZ41uo311Oi_B5vrL3XKVlT--flt-LjOdIzpkvJCWFpYwVNWE4zqvRV7RSmpRS9QUuTU5qwjnpLKyFpTlkhujK0mlrZmmDb0E74--h-DvRxsH1blobNvq3voxKi4QJ1IUCfxwBE3wMQbbqENwnQ6TwkjNJavHkhP75mQ6Vp2tz-Sp1QS8OwE6Gt02IbXk4pnjRIi0ocRlR87FwT486jr8ToNRwdTd-lb9ulrfrMrVT1Um_u2Rb7RXehuS5-aWIEwRkpywgp6TtYlq78eQFhf_84V_2omq5g</recordid><startdate>20041117</startdate><enddate>20041117</enddate><creator>Lodge-Ivey, Shanna L</creator><creator>May, Tammy</creator><creator>Petersen, Mark K</creator><creator>Strickland, James R</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041117</creationdate><title>Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection</title><author>Lodge-Ivey, Shanna L ; May, Tammy ; Petersen, Mark K ; Strickland, James R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a403t-689e38e250bd261d4d74b3b9a7d90f84ec45b2662be9d735496ccab939ed5a3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>analytical methods</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Fluids - chemistry</topic><topic>Cattle</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dietary Proteins - administration & dosage</topic><topic>fluorescence</topic><topic>fluorometry</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>high performance liquid chromatography</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>methylglyoxal</topic><topic>Pyruvaldehyde - analysis</topic><topic>Rumen</topic><topic>rumen fluids</topic><topic>toxic substances</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lodge-Ivey, Shanna L</creatorcontrib><creatorcontrib>May, Tammy</creatorcontrib><creatorcontrib>Petersen, Mark K</creatorcontrib><creatorcontrib>Strickland, James R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lodge-Ivey, Shanna L</au><au>May, Tammy</au><au>Petersen, Mark K</au><au>Strickland, James R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2004-11-17</date><risdate>2004</risdate><volume>52</volume><issue>23</issue><spage>6875</spage><epage>6878</epage><pages>6875-6878</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at −20 °C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 μg/mL was calculated. The recoveries were >80%, and the coefficients of variation were <15%. This method has proven to be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collected from cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced by ruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxal concentration has the potential to be a useful marker to assess ruminal nitrogen status to aid in more accurate diet formulation. Keywords: Methylglyoxal; HPLC; ruminants; nitrogen, carbohydrate</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15537289</pmid><doi>10.1021/jf049736w</doi><tpages>4</tpages></addata></record> |
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| subjects | analytical methods Animals Biological and medical sciences Body Fluids - chemistry Cattle Chromatography, High Pressure Liquid - methods Dietary Proteins - administration & dosage fluorescence fluorometry Food industries Fundamental and applied biological sciences. Psychology General aspects high performance liquid chromatography Methods of analysis, processing and quality control, regulation, standards methylglyoxal Pyruvaldehyde - analysis Rumen rumen fluids toxic substances |
| title | Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection |
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