Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection

There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at −20 °C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 μg/mL was calculated. The recoveries were >80%, and the coefficients of variation were