cDNA cloning and life-cycle stage-specific expression of coronin from Physarum polycephalum

Coronin cDNA was cloned from the plasmodia of Physarum polycephalum. The amino acid sequence deduced from the cDNA was comprised of 449 residues and showed 60% identity to that of Dictyostelium discoideum coronin. Southern blot analysis suggested that the coronin gene present in the P. polycephalum...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-03, Vol.73 (3), p.747-749
Hauptverfasser:	Minami, Y.(Okayama Univ. of Science (Japan)), Ishihara, M, Hayase, M, Sakaguchi, T, Yubisui, T
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Butyrolactone - analogs & derivatives 4-Butyrolactone - chemistry 4-Butyrolactone - genetics ACTIN ACTINA ACTINE ADN Amino Acid Sequence AMINO ACID SEQUENCES Animals BINDING PROTEINS Biological and medical sciences CLONACION CLONAGE CLONING Cloning, Molecular coronin DICTYOSTELIUM DISCOIDEUM DIPLOIDIA DIPLOIDIE DIPLOIDY DNA DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental IDENTIFICACION IDENTIFICATION Life Cycle Stages - genetics life-cycle Molecular Sequence Data PHYSARUM POLYCEPHALUM Physarum polycephalum - genetics Physarum polycephalum - growth & development PROTEINAS AGLUTINANTES PROTEINE DE LIAISON SECUENCIAS DE AMINOACIDOS Sequence Alignment SEQUENCE D'ACIDES AMINES
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Beschreibung
Zusammenfassung:	Coronin cDNA was cloned from the plasmodia of Physarum polycephalum. The amino acid sequence deduced from the cDNA was comprised of 449 residues and showed 60% identity to that of Dictyostelium discoideum coronin. Southern blot analysis suggested that the coronin gene present in the P. polycephalum genome might be a single copy. Coronin was expressed in diploid plasmodia, while it was not detected in haploid amoebae or spores.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.80673