Ecdysteroids and a sucrose phenylpropanoid ester from Froelichia floridana

Phytoecdysteroid glycosides 1– 5 and a phenylpropanoid ester of sucrose 6 were isolated from Froelichia floridana, along with eight known compounds ( 7– 14). Compounds 1– 2 and 6– 14 were tested in vitro for their activity against DNA topoisomerase I, but only diosmetin ( 13) exhibited marginal inhibition. Phytoecdysteroid glycosides ( 1– 5) and a phenylpropanoid ester of sucrose ( 6) were isolated from the whole plant of Froelichia floridana , along with eight known compounds including three ecdysteroids ( 7– 9), four flavonoids ( 10– 13), and one phenolic compound ( 14). Structures were determined using a combination of spectroscopic techniques. Compounds 1, 2 and 6– 14 were tested in vitro for their activity against human DNA topoisomerase I. Compound 13 (diosmetin) showed marginal inhibition against topoisomerase I with IC 50 of 130 μM in conjunction with low intercalation ability.