Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor

This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis sp...

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Veröffentlicht in:	Analyst (London) 2009-04, Vol.134 (4), p.738-742
Hauptverfasser:	WANG, Dian-Bing, BI, Li-Jun, ZHANG, Zhi-Ping, CHEN, Yuan-Yuan, YANG, Rui-Fu, WEI, Hong-Ping, ZHOU, Ya-Feng, ZHANG, Xian-En
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies, Monoclonal - chemistry Antigens, Bacterial - chemistry Bacillus Bacillus anthracis - physiology Biological and medical sciences Biosensors Biotechnology Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Methods. Procedures. Technologies Spores, Bacterial Surface Plasmon Resonance - methods Various methods and equipments
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Beschreibung
Zusammenfassung:	This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/b813038h