Investigation of the silylation of ephedrines using N-methyl-N-trimethylsilyl-trifluoroacetamide

This paper describes a gas chromatography (GC)--mass spectrometry (MS) method for the simultaneous quantification of ephedrine, pseudoephedrine, cathine, norephedrine and methylephedrine in urine. The sample preparation step includes solid phase extraction and derivatisation with N-methyl-N-trimethy...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-11, Vol.811 (2), p.201-208
Hauptverfasser:	FORSDAHL, Guro, GMEINER, Günter
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetamides Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Doping in Sports Ephedrine - analysis Ephedrine - chemistry Fluoroacetates Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry - methods General pharmacology Humans Medical sciences N-Acetylneuraminic Acid - chemistry Pharmacology. Drug treatments Sensitivity and Specificity Trifluoroacetic Acid - chemistry Trimethylsilyl Compounds - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This paper describes a gas chromatography (GC)--mass spectrometry (MS) method for the simultaneous quantification of ephedrine, pseudoephedrine, cathine, norephedrine and methylephedrine in urine. The sample preparation step includes solid phase extraction and derivatisation with N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA). An evaluation of various silylation conditions compatible with screening methods in doping control analysis is presented. The method was found to be well suited for quantification of ephedrines in doping control.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/S1570-0232(04)00704-4