Morphological changes in brain and heart after the temporary liver exclusion from the bloodstream during the cholestasis

The aim of the study was the detection of the peculiarities of the changes in brain and heart, developed by the cholestasis after ischemia (due to the temporary liver exclusion from the bloodstream) and post-ischemic restoration of bloodstream. The experimental study was carried out on 12 mature rats (weight 150-200 g). After the creation of 5 day cholestasis pattern, the exploration and clamping of the hepato-duodenal ligament was done for 10 minutes. After the 15 minutes, 24 hours and 48 hours of the clamp withdrawal from the portal complex, the pieces of the heart and the brain were investigated. The aether narcosis was applied for the anaesthesia purpose. The changes peculiar to the brain damage (perivascular and pericellular swelling, proliferation of glia and neurophagia) were observed shortly after the removal of the ischemia. The mentioned changes were dramatic after 24-48 hours of experimentation. The study suggests that the dramatic changes were developed in the brain and cardiac tissues after 15 min. of the experimentation. Mentioned changes are dismetabolic processes and they continuing in the later terms, including the 48 hours later of the experimentation.