Polyethyleneimine grafted with pluronic P85 enhances Ku86 antisense delivery and the ionizing radiation treatment efficacy in vivo

In an effort to improve the efficacy of antisense delivery, we evaluated polyethyleneimine (PEI, 2 kDa) alone or grafted with nonionic amphiphilic block copolymer Pluronic ® (P85) as a carrier for Ku86 antisense oligonucleotide (ASO) delivery. Ku86 is an abundant nuclear protein that plays an import...

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Veröffentlicht in:	Gene therapy 2004-11, Vol.11 (22), p.1665-1672
Hauptverfasser:	Belenkov, A I, Alakhov, V Y, Kabanov, A V, Vinogradov, S V, Panasci, L C, Monia, B P, Chow, T Y K
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Antigens, Nuclear - genetics Antisense oligonucleotides Antisense therapy Antitumor activity Applied cell therapy and gene therapy Biological and medical sciences Biological products industry Biomedical and Life Sciences Biomedicine Biotechnology Cell Biology Cell death Cell Line, Tumor Computer peripherals industry DNA-Binding Proteins - genetics Drug resistance Female Fundamental and applied biological sciences. Psychology Gene Expression Gene Therapy Genetic Therapy - methods Health. Pharmaceutical industry Human Genetics Humans Industrial applications and implications. Economical aspects Ionizing radiation Ku Autoantigen Medical sciences Mice Mice, Inbred BALB C Morbidity mRNA Nanotechnology Neoplasm Transplantation Neoplasms - therapy Oligonucleotides, Antisense - administration & dosage Poloxalene Polyethyleneimine Protein folding Radiation research-article Transfection Transfection - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation, Heterologous Tumorigenesis Xenografts
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Zusammenfassung:	In an effort to improve the efficacy of antisense delivery, we evaluated polyethyleneimine (PEI, 2 kDa) alone or grafted with nonionic amphiphilic block copolymer Pluronic ® (P85) as a carrier for Ku86 antisense oligonucleotide (ASO) delivery. Ku86 is an abundant nuclear protein that plays an important role in nonhomologous DNA end joining and has implications in tumorigenesis and acquired drug resistance. Transfection of adherent and suspension cell lines with Ku86 ASOs complexed with P85-g-PEI (2 kDa) conjugates was associated with a specific decrease in Ku86 mRNA levels (EC 50
ISSN:	0969-7128 1476-5462
DOI:	10.1038/sj.gt.3302355