An assay of mammalian cell micropermeabilization based on measurements of cellular lactate production
A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precis...
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| Veröffentlicht in: | Analytical biochemistry 2004-11, Vol.334 (2), p.234-238 |
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| container_end_page | 238 |
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| container_issue | 2 |
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| container_title | Analytical biochemistry |
| container_volume | 334 |
| creator | Lehoux, Eric A. Baker, Sharon M. Bush, Jeremy A. Spivey, H. Olin |
| description | A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precision similar to that of micropermeabilization assays based on the release of
86Rb
+ from preloaded cells, avoidance of radioactivity, and simplicity of the measurements and equipment required. |
| doi_str_mv | 10.1016/j.ab.2004.08.007 |
| format | Article |
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86Rb
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86Rb
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86Rb
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| ispartof | Analytical biochemistry, 2004-11, Vol.334 (2), p.234-238 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacterial Toxins - pharmacology Cell Line, Tumor Cell Membrane Permeability - drug effects Glycolysis Lactic Acid - analysis Lactic Acid - biosynthesis Lactic Acid - metabolism Phosphorus - pharmacology Rats Reproducibility of Results Staphylococcus aureus - metabolism |
| title | An assay of mammalian cell micropermeabilization based on measurements of cellular lactate production |
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