An assay of mammalian cell micropermeabilization based on measurements of cellular lactate production

A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precis...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 2004-11, Vol.334 (2), p.234-238
Hauptverfasser:	Lehoux, Eric A., Baker, Sharon M., Bush, Jeremy A., Spivey, H. Olin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - pharmacology Cell Line, Tumor Cell Membrane Permeability - drug effects Glycolysis Lactic Acid - analysis Lactic Acid - biosynthesis Lactic Acid - metabolism Phosphorus - pharmacology Rats Reproducibility of Results Staphylococcus aureus - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precision similar to that of micropermeabilization assays based on the release of 86Rb + from preloaded cells, avoidance of radioactivity, and simplicity of the measurements and equipment required.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2004.08.007