Polyphasic Characterisation of Burkholderia cepacia-Like Isolates Leading to the Emended Description of Burkholderia pyrrocinia

Twenty-five Burkholderia cepacia-like isolates of human and environmental origin, comprising five different recA RFLP types, were examined by using a polyphasic taxonomic approach, including recA gene sequence analysis, 16S rRNA gene sequence analysis, DNA:DNA hybridisation studies, tDNA-PCR, fatty...

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Veröffentlicht in:	Systematic and applied microbiology 2004-09, Vol.27 (5), p.517-526
Hauptverfasser:	Storms, Virginie, Van Den Vreken, Natasja, Coenye, Tom, Mahenthiralingam, Eshwar, LiPuma, John J., Gillis, Monique, Vandamme, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Typing Techniques Bacteriology Biological and medical sciences Burkholderia Burkholderia cepacia Burkholderia cepacia complex Burkholderia cepacia complex - chemistry Burkholderia cepacia complex - classification Burkholderia cepacia complex - genetics Burkholderia cepacia complex - isolation & purification Burkholderia cepacia complex - metabolism Burkholderia Infections - microbiology Burkholderia pyrrocinia Cystic Fibrosis - complications Cystic Fibrosis - microbiology DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Ribosomal - chemistry DNA, Ribosomal - isolation & purification Fatty Acids - analysis Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes, rRNA Humans Microbiology Miscellaneous Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Polymorphism, Restriction Fragment Length polyphasic taxonomy Rec A Recombinases - genetics RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sequence Homology Soil Microbiology
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Zusammenfassung:	Twenty-five Burkholderia cepacia-like isolates of human and environmental origin, comprising five different recA RFLP types, were examined by using a polyphasic taxonomic approach, including recA gene sequence analysis, 16S rRNA gene sequence analysis, DNA:DNA hybridisation studies, tDNA-PCR, fatty acid analysis and biochemical analysis. The results of the present study demonstrated that twenty-three of these strains belong to Burkholderia pyrrocinia, a B. cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis.
ISSN:	0723-2020 1618-0984
DOI:	10.1078/0723202041748190