Action of transglucosidase from Aspergillus niger on maltoheptaose and [U– 13C]maltose
Oligosaccharides synthesized from a mixture of maltoheptaose and [U– 13C]maltose with transglucosidase [EC 2.4.1.24] from Aspergillus niger were investigated. When the reaction mixture was incubated at 15 °C for 1 h, several types of oligosaccharides with DP (degree of polymerization) 2 to DP8 conta...
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Veröffentlicht in: | Carbohydrate research 2009-03, Vol.344 (4), p.460-465 |
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Sprache: | eng |
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Zusammenfassung: | Oligosaccharides synthesized from a mixture of maltoheptaose and [U–
13C]maltose with transglucosidase [EC 2.4.1.24] from
Aspergillus niger were investigated. When the reaction mixture was incubated at 15
°C for 1
h, several types of oligosaccharides with DP (degree of polymerization) 2 to DP8 containing α-
d-Glc
p-(1→6)-maltoheptaose were detected by liquid chromatography–mass spectrometry (LC–MS) and methylation analysis. Most of these compounds consisted of α-(1→4) linkages in the main chain and α-(1→6) linkages at the non-reducing ends. However, when the reaction mixture was incubated for 96
h, most of these products were converted into oligosaccharides with DP2 to DP5 consisting of only α-(1→6) linkages. These results suggested that
A. niger transglucosidase rapidly transferred glucosyl residues to maltooligosaccharides, and gradually hydrolyzed both α-(1→4) linkages and α-(1→6) linkages at the non-reducing end, and transformed these into smaller molecules of mainly α-(1→6) linkages. |
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ISSN: | 0008-6215 1873-426X |
DOI: | 10.1016/j.carres.2008.12.004 |