Indirect immunofluorescence microscopy for direct detection of Xylella fastidiosa in xylem sap
The plant pathogen Xylella fastidiosa is the causative agent of a number of diseases of economically important crops, including Pierces disease that affects grapevines. Using a commercially available antibody specific for X. fastidiosa, we have established a protocol for microscopic identification o...
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Veröffentlicht in: | Current microbiology 2004-11, Vol.49 (5), p.372-375 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The plant pathogen Xylella fastidiosa is the causative agent of a number of diseases of economically important crops, including Pierces disease that affects grapevines. Using a commercially available antibody specific for X. fastidiosa, we have established a protocol for microscopic identification of the bacterium by indirect immunofluorescence. This antibody clearly labels an uncharacterized antigen concentrated at a single pole of X. fastidiosa cells, but does not react with a non-Xylella control. This technique was also performed successfully on xylem exudates from several different plant genera and correlated well with standard enzyme-linked immunosorbent assay tests. These results establish a novel method for in situ assessment of X. fastidiosa infection from host plants. |
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ISSN: | 0343-8651 1432-0991 |
DOI: | 10.1007/s00284-004-4369-5 |