Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis
PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the bio...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2004-10 (20), p.2260-2261 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Cox, Russell J Glod, Frank Hurley, Deirdre Lazarus, Colin M Nicholson, Thomas P Rudd, Brian A M Simpson, Thomas J Wilkinson, Barrie Zhang, Ying |
| description | PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain. |
| doi_str_mv | 10.1039/b411973h |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2004-10 (20), p.2260-2261 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66976000 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Ascomycota - enzymology Ascomycota - genetics Bridged Bicyclo Compounds, Heterocyclic - metabolism DNA, Complementary - biosynthesis DNA, Complementary - genetics Gene Amplification Peptide Library Polyketide Synthases - genetics Promoter Regions, Genetic Tricarboxylic Acids - metabolism |
| title | Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis |
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