Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the bio...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2004-10 (20), p.2260-2261
Hauptverfasser:	Cox, Russell J, Glod, Frank, Hurley, Deirdre, Lazarus, Colin M, Nicholson, Thomas P, Rudd, Brian A M, Simpson, Thomas J, Wilkinson, Barrie, Zhang, Ying
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - enzymology Ascomycota - genetics Bridged Bicyclo Compounds, Heterocyclic - metabolism DNA, Complementary - biosynthesis DNA, Complementary - genetics Gene Amplification Peptide Library Polyketide Synthases - genetics Promoter Regions, Genetic Tricarboxylic Acids - metabolism
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Beschreibung
Zusammenfassung:	PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/b411973h