Heterologously expressed protein phosphatase calcineurin downregulates plant plasma membrane H +-ATPase activity at the post-translational level

To investigate the effects of calcineurin expression on cellular ion homeostasis in plants, we have obtained a transgenic cell culture of tomato, expressing constitutively activated yeast calcineurin. Transgenic cells exhibited reduced growth rates and proton extrusion activity in vivo. We show that...

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Veröffentlicht in:FEBS letters 2004-10, Vol.576 (1), p.266-270
Hauptverfasser: Marı́n-Manzano, Mari Carmen, Rodrı́guez-Rosales, Maria Pilar, Belver, Andrés, Donaire, Juan Pedro, Venema, Kees
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Sprache:eng
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Zusammenfassung:To investigate the effects of calcineurin expression on cellular ion homeostasis in plants, we have obtained a transgenic cell culture of tomato, expressing constitutively activated yeast calcineurin. Transgenic cells exhibited reduced growth rates and proton extrusion activity in vivo. We show that reduction of plasma membrane H +-ATPase activity by expression of calcineurin is the basis for the observed phenotypes. Transgenic calli and cell suspensions displayed also increased salt tolerance and contained slightly higher Ca 2+ and K + levels. This demonstrates that calcineurin can modulate ion homeostasis in plants as it does in yeast by affecting the activity of primary ion transporters.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.09.008