Ghrelin and the differential regulation of des-acyl (DSG) and oct-anoyl ghrelin (OTG) in human adipose tissue (AT)

Summary Objectives Ghrelin, an important central acting orexigenic hormone, is predominantly secreted in the gastrointestinal tract. However little is known about the action of ghrelin in human adipose tissue (AT). Aim To study the expression of ghrelin in AT, the effects of octanoyl–(OTG) and des...

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Veröffentlicht in:	Clinical endocrinology (Oxford) 2009-03, Vol.70 (3), p.383-389
Hauptverfasser:	Kos, K., Harte, A. L., O'Hare, P. J., Kumar, S., McTernan, P. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Adult Arginine - analogs & derivatives Arginine - pharmacology Biological and medical sciences Body Mass Index Cells, Cultured Endocrinopathies Female Fundamental and applied biological sciences. Psychology Ghrelin - metabolism Ghrelin - pharmacology Humans Leptin - metabolism Lipogenesis - drug effects Lipogenesis - physiology Lipolysis - drug effects Lipolysis - physiology Lipoprotein Lipase - metabolism Medical sciences Middle Aged Receptors, Neuropeptide Y - antagonists & inhibitors Subcutaneous Fat - cytology Subcutaneous Fat - drug effects Subcutaneous Fat - metabolism Vertebrates: endocrinology
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Zusammenfassung:	Summary Objectives Ghrelin, an important central acting orexigenic hormone, is predominantly secreted in the gastrointestinal tract. However little is known about the action of ghrelin in human adipose tissue (AT). Aim To study the expression of ghrelin in AT, the effects of octanoyl–(OTG) and des‐acyl (DSG) ghrelin on lipolysis and lipogenesis, leptin release and potential peripheral signalling through the Y1 receptor. Methods Ex vivo human AT was obtained from women undergoing elective surgery (46 (mean ± SD) 6·8 years, body mass index (BMI): 25·6 ± 5·0 kg/m2, n = 20). Abdominal‐subcutaneous (AbdSc) adipocytes were isolated and treated with recombinant human (rh) OTG and DSG to assess lipid metabolism leptin release and the influence of Y1‐receptor blocker. Results Ghrelin was expressed in AbdScAT and negatively correlated with BMI (lean: 3·6 ± 0·74 optical‐density‐units (OD), obese: 1·64 ± 0·45OD, *P
ISSN:	0300-0664 1365-2265
DOI:	10.1111/j.1365-2265.2008.03321.x