Cereblon is expressed in the retina and binds to voltage-gated chloride channels

The function of the central nervous system depends on the correct regulation of ion channels by interacting proteins. Here, we identified cereblon as a new interactor of the voltage-gated chloride channel ClC-2. A distal region of the ClC-2 C-terminus interacts with the Lon domain of cereblon. Cereb...

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Veröffentlicht in:	FEBS letters 2009-02, Vol.583 (4), p.633-637
Hauptverfasser:	Hohberger, Bettina, Enz, Ralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ATP-Dependent Proteases Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism CBS central nervous system Cereblon Chloride Channels - genetics Chloride Channels - metabolism Chloride Channels - physiology Chlorides - metabolism CIC-1 CIC-2 ClC CNS CRBN cystathione b-synthase EF1α elongation factor 1α Escherichia coli - genetics ganglion cell layer GCL glutathione S-transferase Glutathione Transferase - metabolism GST Immunohistochemistry INL inner nuclear layer inner plexiform layer Ion Channel Gating - genetics Ion Channel Gating - physiology IPL Nuclear Proteins - genetics Nuclear Proteins - metabolism ONL OPL outer nuclear layer outer plexiform layer photoreceptor segments Protein Binding Protein interaction Protein Structure, Secondary Protein Structure, Tertiary PRS Recombinant Proteins - metabolism Retina Retina - metabolism Two-Hybrid System Techniques Ubiquitin-Protein Ligase Complexes voltage-gated chloride channel β-Gal β-galactosidase
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Zusammenfassung:	The function of the central nervous system depends on the correct regulation of ion channels by interacting proteins. Here, we identified cereblon as a new interactor of the voltage-gated chloride channel ClC-2. A distal region of the ClC-2 C-terminus interacts with the Lon domain of cereblon. Cereblon is expressed in several brain regions including the retina. There, we detected cereblon in nuclear and synaptic layers and localized the protein in the same subcellular region of bipolar cell bodies previously reported to express ClC-2. Our data suggest that cereblon might be associated with voltage-gated chloride channels in the central nervous system. MINT- 6823070: CIC-2 (uniprotkb: O54822) physically interacts (MI: 0218) with CRBN (uniprotkb: Q0P564) by two hybrid (MI: 0018) MINT- 6823160, MINT- 6823197: CIC-2 (uniprotkb: O54822) physically interacts (MI: 0218) with CRBN (uniprotkb: Q56AP7) by pull down (MI: 0096) MINT- 6823105: CIC-2 (uniprotkb: O54822) physically interacts (MI: 0218) with IK (uniprotkb: A4FUY8) by two hybrid (MI: 0018)
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2009.01.018