The effect of various liposome formulations on insulin penetration across Caco-2 cell monolayer
The aim of the study was to determine the penetration properties of various insulin containing liposome formulations through Caco-2 cell monolayer and to compare the in vitro test results with in vivo tests. The effect of sodium taurocholate as a penetration enhancer when it was added to the liposom...
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Veröffentlicht in: | Life sciences (1973) 2004-10, Vol.75 (23), p.2819-2827 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The aim of the study was to determine the penetration properties of various insulin containing liposome formulations through Caco-2 cell monolayer and to compare the in vitro test results with in vivo tests. The effect of sodium taurocholate as a penetration enhancer when it was added to the liposome formulation was also investigated. In vitro permeation experiments were performed in diffusion cells with the Caco-2 cell monolayer used as the membrane. Permeability values of various insulin containing liposome formulations through Caco-2 cells were determined (log k
insulin-solution = −2.217 ± 0.0723 cm.h
−1, log k
insulin-liposome = −2.141 ± 0.0625 cm.h
−1, log k
insulin-sodium tauroholate liposome= −1.952 ± 0.0623 cm.h
−1). In vivo tests were performed in mice. Formulations were administered orally and blood glucose levels were determined and penetrations were compared with the Caco-2 cell experiment results. In conclusion, the permeability of insulin was increased across Caco-2 cell monolayer when the liposome sodium taurocholate (NaTC) formulation was used. The oral administration of insulin and NaTC incorporated liposomes significantly decreased blood glucose levels. Furthermore, it was shown that a high in vitro/in vivo correlation was observed using the Caco-2 cell monolayer model. |
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ISSN: | 0024-3205 1879-0631 |
DOI: | 10.1016/j.lfs.2004.05.027 |