Determination of azole antifungal medicines using zero-order and derivative UV spectrophotometry

This paper presents a new methodology of quantitative determination of seven azole antifungal medicines widely used in therapy. Analyses were performed directly by using zero-order (fluconazole), first derivative (bifonazole, clotrimazole, econazole, itraconazole, miconazole) and second derivative (ketoconazole) UV spectrophotometry. Validation of all methods confirms their proper precision (%RSD = 0.47-2.86), recovery (98.7-101.4) and linearity (r coefficient over 0,999) in concentrations under investigation. The parameters received enable the developed procedure to be used in quantitative and as auxiliary in qualitative pharmaceutical analysis.