Vibrio gallaecicus sp. nov. isolated from cultured clams in north-western Spain

A group of three motile facultative anaerobic marine bacteria were isolated from cultured Manila clams ( Ruditapes philippinarum) in Galicia, north-western Spain. The strains were characterized phenotypically and genotypically. Phylogenetic analysis of the 16S rRNA gene and four housekeeping genes,...

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Veröffentlicht in:	Systematic and applied microbiology 2009-04, Vol.32 (2), p.111-117
Hauptverfasser:	Beaz-Hidalgo, Roxana, Doce, Alejandra, Pascual, Javier, Toranzo, Alicia E., Romalde, Jesús L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal aquaculture Animal productions Animals Aquaculture Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Typing Techniques Bacteriology Biological and medical sciences Bivalvia - microbiology DNA, Bacterial - analysis DNA, Ribosomal - analysis Fundamental and applied biological sciences. Psychology Genes, rRNA Invertebrate aquaculture Marine Microbiology MLSA Molecular Sequence Data Mollusca Nucleic Acid Hybridization Phenotype Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Ruditapes philippinarum Sequence Analysis, DNA Spain Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Systematics Vibrio Vibrio - classification Vibrio - genetics Vibrio - isolation & purification Vibrio gallaecicus sp. nov Vibrio splendidus Vibrio splendidus clade
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Zusammenfassung:	A group of three motile facultative anaerobic marine bacteria were isolated from cultured Manila clams ( Ruditapes philippinarum) in Galicia, north-western Spain. The strains were characterized phenotypically and genotypically. Phylogenetic analysis of the 16S rRNA gene and four housekeeping genes, RNA polymerase α-chain ( rpoA), RecA protein ( recA), the α-subunit of bacterial ATP synthase ( atpA) and the uridine monophosphate (UMP) kinase ( pyrH), indicated that these strains were closely related to the Vibrio splendidus clade. The amplified fragment length polymorphism (AFLP) fingerprints, DNA–DNA hybridizations and phylogenies of the housekeeping and 16S rRNA gene sequences showed that the three strains represented a different species from all currently described vibrios. The new species could be differentiated from its nearest neighbours on the basis of several phenotypic features. The three strains are therefore a novel species within the genus Vibrio, for which the name Vibrio gallaecicus is proposed, with the type strain being VB 8.9 T(=CECT 7244 T=LMG 24045 T).
ISSN:	0723-2020 1618-0984
DOI:	10.1016/j.syapm.2008.12.002