Infrared Atmospheric Pressure MALDI Ion Trap Mass Spectrometry of Frozen Samples Using a Peltier-Cooled Sample Stage

Infrared Atmospheric Pressure MALDI Ion Trap Mass Spectrometry of Frozen Samples Using a Peltier-Cooled Sample Stage

Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to analyze frozen samples generated using a Peltier-cooled sample stage. This allows for the analysis of samples in water without the addition of matrix, in near-native conditions, and...

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Veröffentlicht in:Analytical chemistry (Washington) 2004-10, Vol.76 (19), p.5887-5893
Hauptverfasser: Von Seggern, Christopher E, Gardner, Ben D, Cotter, Robert J
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Analytical chemistry
Atmospheric Pressure
Carbohydrate Conformation
Carbohydrates
Chemistry
Cold Temperature
Enkephalin, Leucine - chemistry
Exact sciences and technology
Freezing
Gastrins - chemistry
Infrared Rays
Ions - chemistry
Lactose - chemistry
Lipids
Mass spectrometry
Molecular Sequence Data
Peptides
Specimen Handling - methods
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Zusammenfassung:Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to analyze frozen samples generated using a Peltier-cooled sample stage. This allows for the analysis of samples in water without the addition of matrix, in near-native conditions, and with minimal loss of water due to evaporation. Analysis of frozen samples is extended to study peptides, carbohydrates, and glycolipids.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac049575v