Ultrasensitive Electrocatalytic DNA Detection at Two- and Three-Dimensional Nanoelectrodes

Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electro...

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Veröffentlicht in:	Journal of the American Chemical Society 2004-10, Vol.126 (39), p.12270-12271
Hauptverfasser:	Gasparac, Rahela, Taft, Bradford J, Lapierre-Devlin, Melissa A, Lazareck, Adam D, Xu, Jimmy M, Kelley, Shana O
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Catalysis Cations - chemistry DNA - analysis DNA - chemistry DNA - genetics Electrochemistry - methods Ferric Compounds - chemistry Fundamental and applied biological sciences. Psychology Gold - chemistry Helicobacter pylori - genetics Methods. Procedures. Technologies Microelectrodes Microscopy, Electron, Scanning Nanotechnology - methods Nucleic Acid Hybridization Oligonucleotides - chemistry Oligonucleotides - genetics RNA, Ribosomal, 23S - analysis RNA, Ribosomal, 23S - genetics Ruthenium - chemistry Sensitivity and Specificity Various methods and equipments
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Beschreibung
Zusammenfassung:	Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electrochemical sensing. High-sensitivity DNA detection was achieved at oligonucleotide-functionalized NEEs using a label-free electrocatalytic assay. Attomole levels of DNA were detected using the NEEs, validating the promise of nanoarchitectures for ultrasensitive biosensing.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja0458221