Triple-Resonance Coherent Anti-Stokes Raman Scattering Microspectroscopy

Fluorescence‐free microscopy: A new nonlinear optical microspectroscopy technique, femtosecond (fs) triple‐resonance coherent anti‐Stokes Raman scattering, in which the amplitude and phase of input fs laser pulses are optimally shaped to be in triple resonance with the molecular electronic and vibrational transitions, generates a coherent nonlinear signal beam at a new color with a highest possible efficiency (see figure). Fluorescence‐free microscopy: A new nonlinear optical microspectroscopy technique, femtosecond (fs) triple‐resonance coherent anti‐Stokes Raman scattering, in which the amplitude and phase of input fs laser pulses are optimally shaped to be in triple resonance with the molecular electronic and vibrational transitions, generates a coherent nonlinear signal beam at a new color with a highest possible efficiency (see figure).