Proton-Detected Solid-State NMR Spectroscopy of Natural-Abundance Peptide and Protein Pharmaceuticals

The natural way: A sensitive NMR spectroscopic method is developed to obtain well-resolved two-dimensional spectra (¹⁵N-¹H and ¹³C-¹H) for natural-abundance (that is, without the need for isotopic enrichment) large-molecule samples, such as biopharmaceuticals. This method gives structural insights o...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2009-02, Vol.48 (7), p.1253-1256
Hauptverfasser:	Zhou, Donghua H, Shah, Gautam, Mullen, Charles, Sandoz, Dennis, Rienstra, Chad M
Format:	Artikel
Sprache:	eng
Schlagworte:	Aprotinin - chemistry Freeze Drying Humans insulin Insulin - chemistry magic-angle spinning Molecular Structure NMR spectroscopy Nuclear Magnetic Resonance, Biomolecular - methods Peptides - chemistry proteins Proteins - chemistry Protons structure elucidation
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Beschreibung
Zusammenfassung:	The natural way: A sensitive NMR spectroscopic method is developed to obtain well-resolved two-dimensional spectra (¹⁵N-¹H and ¹³C-¹H) for natural-abundance (that is, without the need for isotopic enrichment) large-molecule samples, such as biopharmaceuticals. This method gives structural insights on two lyophilized aprotinin samples and three insulin samples in lyophilized, microcrystalline suspension formulation (red; see picture) and fibril (green) forms.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200801029