Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se Received February 18, 2004 Accepted June 25, 2004 A phage display library made from Staphylococcus aureus DNA was sorted against a central ve...
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| Veröffentlicht in: | Journal of medical microbiology 2004-10, Vol.53 (10), p.945-951 |
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| container_title | Journal of medical microbiology |
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| creator | Bjerketorp, Joakim Rosander, Anna Nilsson, Martin Jacobsson, Karin Frykberg, Lars |
| description | Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se
Received February 18, 2004
Accepted June 25, 2004
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß 2 -glycoprotein I (ß 2 -GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß 2 -GPI was also detected on all tested CVCs.
Abbreviations: CF, clumping factor; CVC, central venous catheter; Fg, fibrinogen; Fn, fibronectin; ß 2 -GPI, ß 2 -glycoprotein I. |
| doi_str_mv | 10.1099/jmm.0.45638-0 |
| format | Article |
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Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se
Received February 18, 2004
Accepted June 25, 2004
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß 2 -glycoprotein I (ß 2 -GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß 2 -GPI was also detected on all tested CVCs.
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Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se
Received February 18, 2004
Accepted June 25, 2004
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß 2 -glycoprotein I (ß 2 -GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß 2 -GPI was also detected on all tested CVCs.
Abbreviations: CF, clumping factor; CVC, central venous catheter; Fg, fibrinogen; Fn, fibronectin; ß 2 -GPI, ß 2 -glycoprotein I.</description><subject>Bacterial Adhesion</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>beta 2-Glycoprotein I</subject><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - metabolism</subject><subject>Catheterization, Central Venous</subject><subject>Fibrinogen - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - metabolism</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Peptide Library</subject><subject>Protein Binding</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - physiology</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1v2zAQgGGiSNA4aceuBZcUXeTwW9JYGGkTIEBQJJ2JE0VKDPRVUnLif1-6NuAx0y0PjscXoS-UrCkpy5uXvl-TtZCKFxn5gFZU5DyTSogztCKEsYwpKi_QZYwvhNCc8_IjuqCSy6KgcoV-P41h9kODAT_NMLW7bjSjMUvEsASbxtRCY3Ht49TBDne-ChB2GBrwQ5yxfcNbvx1x5cceZhs8dJ_QuYMu2s_HeYX-_Lx93txlD4-_7jc_HjIjGJszW-eG1rwmpVBEMEtk5QzQUjlbcCGMlSKvXCmF45IBuFIVAJWhhkKuHMv5Ffp22DuF8e9i46x7H43tOhjsuEStVKG4oO9DmudKUCkTzA7QhDHGYJ2egu_TdzUleh9bp9ia6P-xNUn-63HxUvW2Pulj3QSujwCigc4FGIyPJ6eoUIqJ5L4fXOub9tUHqxs79D6dkbruH5V8f0IpJP8HSW-WtA</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Bjerketorp, Joakim</creator><creator>Rosander, Anna</creator><creator>Nilsson, Martin</creator><creator>Jacobsson, Karin</creator><creator>Frykberg, Lars</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial</title><author>Bjerketorp, Joakim ; Rosander, Anna ; Nilsson, Martin ; Jacobsson, Karin ; Frykberg, Lars</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-ed7c1d3d0946042e05bfca196fe8344ce547bf954f352aaf968aabc1c1a76f273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bacterial Adhesion</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>beta 2-Glycoprotein I</topic><topic>Biocompatible Materials</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - metabolism</topic><topic>Catheterization, Central Venous</topic><topic>Fibrinogen - metabolism</topic><topic>Fundamental and applied biological sciences. 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Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se
Received February 18, 2004
Accepted June 25, 2004
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß 2 -glycoprotein I (ß 2 -GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß 2 -GPI was also detected on all tested CVCs.
Abbreviations: CF, clumping factor; CVC, central venous catheter; Fg, fibrinogen; Fn, fibronectin; ß 2 -GPI, ß 2 -glycoprotein I.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>15358815</pmid><doi>10.1099/jmm.0.45638-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Bacterial Adhesion Bacterial Proteins - metabolism Bacteriology beta 2-Glycoprotein I Biocompatible Materials Biological and medical sciences Carrier Proteins - metabolism Catheterization, Central Venous Fibrinogen - metabolism Fundamental and applied biological sciences. Psychology Glycoproteins - metabolism Infectious diseases Medical sciences Microbiology Miscellaneous Peptide Library Protein Binding Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - physiology |
| title | Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial |
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