Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se Received February 18, 2004 Accepted June 25, 2004 A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß 2 -glycoprotein I (ß 2 -GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß 2 -GPI was also detected on all tested CVCs. Abbreviations: CF, clumping factor; CVC, central venous catheter; Fg, fibrinogen; Fn, fibronectin; ß 2 -GPI, ß 2 -glycoprotein I.