Synthesis and Triplex Formation of Oligonucleotides Containing 8-Thioxodeoxyadenosine

For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides containing 8-thioxodeoxyadenosine (s8dA) residues in place of the protonated deoxycytidines required for the third base pairing with DNA duplexes. Consequently, it was found that s8dA e...

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Veröffentlicht in:	Organic letters 2009-02, Vol.11 (3), p.605-608
Hauptverfasser:	Miyata, Ken-ichi, Tamamushi, Ryuji, Tsunoda, Hirosuke, Ohkubo, Akihiro, Seio, Kohji, Sekine, Mitsuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Deoxyadenosines - chemical synthesis Deoxyadenosines - chemistry DNA - chemistry Molecular Structure Oligodeoxyribonucleotides - chemical synthesis Oligodeoxyribonucleotides - chemistry Organothiophosphorus Compounds - chemical synthesis Organothiophosphorus Compounds - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Zusammenfassung:	For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides containing 8-thioxodeoxyadenosine (s8dA) residues in place of the protonated deoxycytidines required for the third base pairing with DNA duplexes. Consequently, it was found that s8dA exhibited much stronger hybridization ability than dC under neutral conditions when four s8dA bases were arranged in a consecutive sequence.
ISSN:	1523-7060 1523-7052
DOI:	10.1021/ol802622s