Alteration of the Parasite Plasma Membrane and the Parasitophorous Vacuole Membrane during Exo-Erythrocytic Development of Malaria Parasites
The rodent malaria parasite Plasmodium berghei develops in hepatocytes within 48–52 h from a single sporozoite into up to 20,000 daughter parasites, so-called merozoites. The cellular and molecular details of this extensive proliferation are still largely unknown. Here we have used a transgenic, RFP...
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Veröffentlicht in: | Protist 2009-02, Vol.160 (1), p.51-63 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The rodent malaria parasite
Plasmodium berghei develops in hepatocytes within 48–52
h from a single sporozoite into up to 20,000 daughter parasites, so-called merozoites. The cellular and molecular details of this extensive proliferation are still largely unknown. Here we have used a transgenic, RFP-expressing
P. berghei parasite line and molecular imaging techniques including intravital microscopy to decipher various aspects of parasite development within the hepatocyte. In late schizont stages, MSP1 is expressed and incorporated into the parasite plasma membrane that finally forms the membrane of developing merozoites by continuous invagination steps. We provide first evidence for activation of a verapamil-sensitive Ca
2+ channel in the plasma membrane of liver stage parasites before invagination occurs. During merozoite formation, the permeability of the parasitophorous vacuole membrane changes considerably before it finally becomes completely disrupted, releasing merozoites into the host cell cytoplasm. |
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ISSN: | 1434-4610 1618-0941 |
DOI: | 10.1016/j.protis.2008.08.002 |