Diversity within Borrelia burgdorferi sensu lato genospecies in Switzerland by recA gene sequence

A total of 874 Ixodes ricinus ticks were collected in Switzerland to investigate the genetic diversity of the Borrelia population. We integrated to the RT-PCR method the DNA sequence analysis of a 162-bp fragment of the recA gene. Five genospecies were detected: Borrelia afzelii, Borrelia burgdorfer...

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Veröffentlicht in:	FEMS microbiology letters 2004-09, Vol.238 (1), p.115-123
Hauptverfasser:	Casati, S, Bernasconi, V, Gern, L, Piffaretti, J.C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriology biodiversity Biological and medical sciences Borrelia Borrelia afzelii Borrelia burgdorferi Borrelia burgdorferi Group - classification Borrelia burgdorferi Group - genetics Borrelia burgdorferi Group - isolation & purification Borrelia burgdorferi sensu lato Cats Cattle Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification Dogs Evolution Fundamental and applied biological sciences. Psychology Genetic diversity Genetic Variation Genetics Goats Humans Ixodes - microbiology Ixodes ricinus Larva - microbiology Microbiology Molecular Epidemiology Molecular Sequence Data Nucleotide sequence Nymph - microbiology Phylogeny Polymerase chain reaction Rec A Recombinases - genetics recA RecA protein Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology Switzerland Systematics Ticks
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Beschreibung
Zusammenfassung:	A total of 874 Ixodes ricinus ticks were collected in Switzerland to investigate the genetic diversity of the Borrelia population. We integrated to the RT-PCR method the DNA sequence analysis of a 162-bp fragment of the recA gene. Five genospecies were detected: Borrelia afzelii, Borrelia burgdorferi s.s., Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. A heterogeneous distribution was observed within the B. burgdorferi s.l. genospecies. The most prevalent and diverse genospecies found in Switzerland was Borrelia afzelii, which might suggest a rapid evolution of this genospecies.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.2004.tb09745.x