Association of K-ras Mutations with Liver Metastases from Colorectal Carcinoma
Background: Colorectal carcinomas were studied regarding early proof of liver metastases through determination of K-ras mutations. Patients and Methods: Seventy-seven colorectal carcinomas were investigated for the presence of point mutations in codon 12 and 13 of the K-ras gene, using single-strand...
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Veröffentlicht in: | Anticancer research 2004-07, Vol.24 (4), p.2471-2476 |
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Sprache: | eng |
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Zusammenfassung: | Background: Colorectal carcinomas were studied regarding early proof of liver metastases through determination of K-ras mutations.
Patients and Methods: Seventy-seven colorectal carcinomas were investigated for the presence of point mutations in codon 12
and 13 of the K-ras gene, using single-strand conformation polymorphism (SSCP) analysis and direct sequencing. Results: Twenty-six
carcinomas were positive for K-ras mutations, of which 21 had codon 12 and 5 had codon 13 mutations. Twenty patients with
K-ras-positive tumor (20 out of 26: 77%) developed liver metastases, of which 13 had simultaneous metastases and 7 had metachronous
metastases. There was a significant association between K-ras mutations and liver metastases (p=0.03). A multivariate logistic
regression model demonstrated that the involvement of lymph node (p |
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ISSN: | 0250-7005 1791-7530 |