Activation and Tuning of Green Fluorescent Protein Chromophore Emission by Alkyl Substituent-Mediated Crystal Packing

O-Alkyl synthetic analogues of the green fluorescent protein chromophore are nonfluorescent in solution but demonstrate a bright emission in the crystalline state. Three-dimensional steady-state and time-resolved emission spectroscopies in the solid state, as well as single-crystal X-ray diffraction...

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Veröffentlicht in:	Journal of the American Chemical Society 2009-01, Vol.131 (2), p.662-670
Hauptverfasser:	Dong, Jian, Solntsev, Kyril M, Tolbert, Laren M
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzylidene Compounds - chemistry Crystallization Crystallography, X-Ray Green Fluorescent Proteins - chemistry Imidazolines - chemistry Luminescent Measurements - methods Models, Molecular Photochemical Processes Spectrometry, Fluorescence Structure-Activity Relationship
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	O-Alkyl synthetic analogues of the green fluorescent protein chromophore are nonfluorescent in solution but demonstrate a bright emission in the crystalline state. Three-dimensional steady-state and time-resolved emission spectroscopies in the solid state, as well as single-crystal X-ray diffraction, reveal the nature of complex emission in the crystals. A hypsochromic emission shift with an increase of the alkyl size is determined by the monomer-aggregate emission ratio.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja806962e