Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery
Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topog...
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| Veröffentlicht in: | Gene therapy 2009-01, Vol.16 (1), p.78-92 |
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| creator | Agrawal, V K Copeland, K M Barbachano, Y Rahim, A Seth, R White, C L Hingorani, M Nutting, C M Kelly, M Harris, P Pandha, H Melcher, A A Vile, R G Porter, C Harrington, K J |
| description | Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topography and duration of luciferase gene expression after plasmid and adenoviral delivery in rat superficial inferior epigastric (SIE) flaps. For plasmid delivery, luciferase expression was significantly increased by all transduction routes (topical, intraflap injection, intravascular) (
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| doi_str_mv | 10.1038/gt.2008.140 |
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P
<0.01) at day 1, but not at day 7. The spread of luciferase expression was significantly different between the 4 groups at 1 day (
P
=0.026) and was greatest for flaps transduced by intravascular injection. For adenoviral transduction, total radiance was significantly different between the transduced groups at 1, 14 and 28 days (
P
<0.05 for all comparisons). The highest levels of radiance were seen in the intravascular group. There was a statistically significant difference in the spread of light emission between the 3 groups at 1 (
P
=0.009) and 14 (
P
=0.013) days, but this was no longer evident at 28 days. Intravascular adenoviral delivery yields high-level, diffuse and durable gene expression in rat SIE flaps and is suitable for examination in therapeutic models.</description><identifier>ISSN: 0969-7128</identifier><identifier>EISSN: 1476-5462</identifier><identifier>DOI: 10.1038/gt.2008.140</identifier><identifier>PMID: 18784747</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adenoviridae - genetics ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Applied cell therapy and gene therapy ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Cancer ; Care and treatment ; Cell Biology ; Comparative studies ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Therapy ; Genetic aspects ; Genetic Therapy - methods ; Genetic transformation ; Genetic vectors ; Genetic Vectors - administration & dosage ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; Health aspects ; Health. Pharmaceutical industry ; Human Genetics ; Industrial applications and implications. Economical aspects ; Injections ; Lac Operon ; Luciferases - analysis ; Luciferases - genetics ; Male ; Medical sciences ; Methods ; Models, Animal ; Nanotechnology ; original-article ; Plasmids - pharmacology ; Rats ; Rats, Inbred F344 ; Reverse Transcriptase Polymerase Chain Reaction ; Surgery ; Surgical Flaps ; Tissue Distribution ; Tissues ; Transduction, Genetic - methods ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Gene therapy, 2009-01, Vol.16 (1), p.78-92</ispartof><rights>Macmillan Publishers Limited 2009</rights><rights>2009 INIST-CNRS</rights><rights>COPYRIGHT 2009 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-cbdd8fd9f8d7dc1a7de1e3f952d023e3018f5e1809c0762bd6f4b2db66fa28a43</citedby><cites>FETCH-LOGICAL-c513t-cbdd8fd9f8d7dc1a7de1e3f952d023e3018f5e1809c0762bd6f4b2db66fa28a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21066307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18784747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agrawal, V K</creatorcontrib><creatorcontrib>Copeland, K M</creatorcontrib><creatorcontrib>Barbachano, Y</creatorcontrib><creatorcontrib>Rahim, A</creatorcontrib><creatorcontrib>Seth, R</creatorcontrib><creatorcontrib>White, C L</creatorcontrib><creatorcontrib>Hingorani, M</creatorcontrib><creatorcontrib>Nutting, C M</creatorcontrib><creatorcontrib>Kelly, M</creatorcontrib><creatorcontrib>Harris, P</creatorcontrib><creatorcontrib>Pandha, H</creatorcontrib><creatorcontrib>Melcher, A A</creatorcontrib><creatorcontrib>Vile, R G</creatorcontrib><creatorcontrib>Porter, C</creatorcontrib><creatorcontrib>Harrington, K J</creatorcontrib><title>Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery</title><title>Gene therapy</title><addtitle>Gene Ther</addtitle><addtitle>Gene Ther</addtitle><description>Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topography and duration of luciferase gene expression after plasmid and adenoviral delivery in rat superficial inferior epigastric (SIE) flaps. For plasmid delivery, luciferase expression was significantly increased by all transduction routes (topical, intraflap injection, intravascular) (
P
<0.01) at day 1, but not at day 7. The spread of luciferase expression was significantly different between the 4 groups at 1 day (
P
=0.026) and was greatest for flaps transduced by intravascular injection. For adenoviral transduction, total radiance was significantly different between the transduced groups at 1, 14 and 28 days (
P
<0.05 for all comparisons). The highest levels of radiance were seen in the intravascular group. There was a statistically significant difference in the spread of light emission between the 3 groups at 1 (
P
=0.009) and 14 (
P
=0.013) days, but this was no longer evident at 28 days. Intravascular adenoviral delivery yields high-level, diffuse and durable gene expression in rat SIE flaps and is suitable for examination in therapeutic models.</description><subject>Adenoviridae - genetics</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Applied cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Cell Biology</subject><subject>Comparative studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Therapy</subject><subject>Genetic aspects</subject><subject>Genetic Therapy - methods</subject><subject>Genetic transformation</subject><subject>Genetic vectors</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Health aspects</subject><subject>Health. Pharmaceutical industry</subject><subject>Human Genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Injections</subject><subject>Lac Operon</subject><subject>Luciferases - analysis</subject><subject>Luciferases - genetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Models, Animal</subject><subject>Nanotechnology</subject><subject>original-article</subject><subject>Plasmids - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Surgery</subject><subject>Surgical Flaps</subject><subject>Tissue Distribution</subject><subject>Tissues</subject><subject>Transduction, Genetic - methods</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0969-7128</issn><issn>1476-5462</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpt0l1r2zAUBmAxNtYs29Xuh9hYYWzJJNmx5N2Vso9Cx2Af10KxjlwVRUol2bRX_euTSWiXURsjOH4k2UcvQi8pWVJSiY99XjJCxJLW5BGa0Zo3i1XdsMdoRtqmXXDKxBF6ltIlIaTmgj1FR1RwUfOaz9Dtd9vFMKrUDU5FbCIAzjaloQxR-WSgFEPEPXjAGpwdId58wtbj0Y4Bw6jcoLINHgeDtTXFg884hiFDmmpbp9LGaqx8eTT4MNqo3N1Sz9ETo1yCF_txjv58-fz79Nvi_MfXs9OT80W3olVedGuthdGtEZrrjiqugUJl2hXThFVQESrMCqggbUd4w9a6MfWa6XXTGMWEqqs5Ot6tu43haoCU5camDpxTHsKQZNPwVpBqgq__g5dhiL58m2SlbVRU5ZqjNzvUKwfSehNKs7ppRXlCW1ZRwjkravmAKreGje2CB2NL_WDCu4MJxWS4zr0aUpJnv34e2uN_7AUoly9ScMN0FukQvt_Bcs4pRTByG-1GxRtJiZwCJPsspwDJEqCiX-1_f1hvQN_bfWIKeLsHJTPKmRKSzqY7xyhpmopM7sPOpfLK9xDv-_jQvn8B_nLbUA</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Agrawal, V K</creator><creator>Copeland, K M</creator><creator>Barbachano, Y</creator><creator>Rahim, A</creator><creator>Seth, R</creator><creator>White, C L</creator><creator>Hingorani, M</creator><creator>Nutting, C M</creator><creator>Kelly, M</creator><creator>Harris, P</creator><creator>Pandha, H</creator><creator>Melcher, A A</creator><creator>Vile, R G</creator><creator>Porter, C</creator><creator>Harrington, K J</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery</title><author>Agrawal, V K ; Copeland, K M ; Barbachano, Y ; Rahim, A ; Seth, R ; White, C L ; Hingorani, M ; Nutting, C M ; Kelly, M ; Harris, P ; Pandha, H ; Melcher, A A ; Vile, R G ; Porter, C ; Harrington, K J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-cbdd8fd9f8d7dc1a7de1e3f952d023e3018f5e1809c0762bd6f4b2db66fa28a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenoviridae - genetics</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Applied cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Cell Biology</topic><topic>Comparative studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Therapy</topic><topic>Genetic aspects</topic><topic>Genetic Therapy - methods</topic><topic>Genetic transformation</topic><topic>Genetic vectors</topic><topic>Genetic Vectors - administration & dosage</topic><topic>Genetic Vectors - genetics</topic><topic>Genetic Vectors - metabolism</topic><topic>Health aspects</topic><topic>Health. Pharmaceutical industry</topic><topic>Human Genetics</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Injections</topic><topic>Lac Operon</topic><topic>Luciferases - analysis</topic><topic>Luciferases - genetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Models, Animal</topic><topic>Nanotechnology</topic><topic>original-article</topic><topic>Plasmids - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Surgery</topic><topic>Surgical Flaps</topic><topic>Tissue Distribution</topic><topic>Tissues</topic><topic>Transduction, Genetic - methods</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Agrawal, V K</creatorcontrib><creatorcontrib>Copeland, K M</creatorcontrib><creatorcontrib>Barbachano, Y</creatorcontrib><creatorcontrib>Rahim, A</creatorcontrib><creatorcontrib>Seth, R</creatorcontrib><creatorcontrib>White, C L</creatorcontrib><creatorcontrib>Hingorani, M</creatorcontrib><creatorcontrib>Nutting, C M</creatorcontrib><creatorcontrib>Kelly, M</creatorcontrib><creatorcontrib>Harris, P</creatorcontrib><creatorcontrib>Pandha, H</creatorcontrib><creatorcontrib>Melcher, A A</creatorcontrib><creatorcontrib>Vile, R G</creatorcontrib><creatorcontrib>Porter, C</creatorcontrib><creatorcontrib>Harrington, K J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agrawal, V K</au><au>Copeland, K M</au><au>Barbachano, Y</au><au>Rahim, A</au><au>Seth, R</au><au>White, C L</au><au>Hingorani, M</au><au>Nutting, C M</au><au>Kelly, M</au><au>Harris, P</au><au>Pandha, H</au><au>Melcher, A A</au><au>Vile, R G</au><au>Porter, C</au><au>Harrington, K J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery</atitle><jtitle>Gene therapy</jtitle><stitle>Gene Ther</stitle><addtitle>Gene Ther</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>16</volume><issue>1</issue><spage>78</spage><epage>92</epage><pages>78-92</pages><issn>0969-7128</issn><eissn>1476-5462</eissn><abstract>Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topography and duration of luciferase gene expression after plasmid and adenoviral delivery in rat superficial inferior epigastric (SIE) flaps. For plasmid delivery, luciferase expression was significantly increased by all transduction routes (topical, intraflap injection, intravascular) (
P
<0.01) at day 1, but not at day 7. The spread of luciferase expression was significantly different between the 4 groups at 1 day (
P
=0.026) and was greatest for flaps transduced by intravascular injection. For adenoviral transduction, total radiance was significantly different between the transduced groups at 1, 14 and 28 days (
P
<0.05 for all comparisons). The highest levels of radiance were seen in the intravascular group. There was a statistically significant difference in the spread of light emission between the 3 groups at 1 (
P
=0.009) and 14 (
P
=0.013) days, but this was no longer evident at 28 days. Intravascular adenoviral delivery yields high-level, diffuse and durable gene expression in rat SIE flaps and is suitable for examination in therapeutic models.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18784747</pmid><doi>10.1038/gt.2008.140</doi><tpages>15</tpages></addata></record> |
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| identifier | ISSN: 0969-7128 |
| ispartof | Gene therapy, 2009-01, Vol.16 (1), p.78-92 |
| issn | 0969-7128 1476-5462 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenoviridae - genetics Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Applied cell therapy and gene therapy Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Cancer Care and treatment Cell Biology Comparative studies Fundamental and applied biological sciences. Psychology Gene Expression Gene Therapy Genetic aspects Genetic Therapy - methods Genetic transformation Genetic vectors Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - metabolism Health aspects Health. Pharmaceutical industry Human Genetics Industrial applications and implications. Economical aspects Injections Lac Operon Luciferases - analysis Luciferases - genetics Male Medical sciences Methods Models, Animal Nanotechnology original-article Plasmids - pharmacology Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Surgery Surgical Flaps Tissue Distribution Tissues Transduction, Genetic - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery |
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